Index Catalog // ScholarsArchive@OSU

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Creator:: Weber, Shane Crawford
Abstract:: I examined, in three separate studies, the active chromatin structure of the yeast, Saccharomyces cerevisiae. Yeast contains four proteins having amino acid compositions typical of the high mobility group (HMG) proteins. Three of these are eluted from chromatin by 0.35 M NaCl; one is not, but is eluted by 0.25...
Resource Type:: Dissertation
Full Text:: are enriched for active genes (Levy-W. & Dixon, 1978a; Levy-W. et al., 1979a; Goodwin, at al., 1981

Hydrogen-stimulated CO₂ fixation and coordinate derepression of hydrogenase and ribulosebisphosphate carboxylase in a H₂-uptake positive strain of Rhizobium japonicum

Creator:: Simpson, Frank Berry
Abstract:: H₂-uptake positive strains (122 DES and SR) and H₂-uptake negative strains SR2 and SR3 of Rhizobium japonicum were examined for ribulosebisphosphate (RuBP) carboxylase and H₂-uptake activities during growth conditions which induced formation of the hydrogenase system. The rate of ¹⁴CO₂ uptake by hydrogenase-induced cells was about six times greater in...
Resource Type:: Masters Thesis
Full Text:: hydrogenase activity in free-living R. japonicum has been accomplished only re- cently (Maier et al., 1978a

Regulation of phosphofructokinase by reversible inactivation

Creator:: Zhao, Zhizhuang
Abstract:: The interaction of skeletal muscle phosphofructokinase with a variety of acidic proteins including calmodulin and troponin C and with nucleic acids (RNA and DNA) is manifest in a reversible, time-dependent loss of catalytic activity. The inactivation is affected by a number of factors. Substrates (fructose-6-phosphate and ATP), positive effectors (fructose-2,6-bisphosphate,...
Resource Type:: Dissertation
Full Text:: fractionation on Sephadex G-75 column were performed according to Leavis et al. (1978a). The purities of all

Metabolic pathway of the nitrosoureas catalyzed by cytochrome P-450 and NADPH cytochrome P-450 reductase

Creator:: Potter, David Willard
Abstract:: Three highly purified forms of cytochrome P-450 (P-450a, P-450b, P-450c) from Aroclor 1254-induced rat hepatic microsomes catalyzed the monooxygenation of 1-(2- chloroethyl)-3-(trans-4-methylcyclohexyl)-1-nitrosourea (MeCCNU) and 1-(2-chloroethyl)-3-(cyclohexyl)-1-nitrosourea (CCNU) in the presence of purified NADPH cytochrome P-450 reductase, NADPH and oxygen. Cytochrome P -450- dependent monooxygenation of CCNU gave six alicyclic hydroxylation products...
Resource Type:: Dissertation

The renaturation of bovine muscle pyruvate kinase

Creator:: Porter, David Hamilton
Resource Type:: Dissertation
Full Text:: unfolded state of RNAase A and other proteins ( Brandts et al., 1975; Schmid and Baldwin, 1978a; Garel and

In vitro transcription in the yeast: Saccharomyces cerevisiae

Creator:: Ide, Gregory James
Abstract:: The structure and transcriptional activity of intranuclear and isolated chromatin from logarithmically growing yeast cells has been compared to chromatin from cells which have entered the stationary phase and ceased growing. Both chromatins show a similar nucleosomal repeat pattern and a 160 by repeat size when digested with staphlococcal nuclease....
Resource Type:: Dissertation

Physical structure and reconstitution of chromatin core particles

Creator:: Tatchell, Kelly George
Abstract:: The nucleosome appears to be the basic structural unit of the eukaryotic chromosome. The usual nucleosome preparation, isolated from a micrococcal nuclease digest of nuclei, is heterogenous in DNA length and protein composition with some particles containing spacer DNA and associated protein. This heterogeneity complicated physical and reconstitution studies. As...
Resource Type:: Dissertation

Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

Creator:: Zhong, Lingxiu
Resource Type:: Dissertation
Full Text:: of the most popular prediction methods is proposed by Chou and Fasman (1974a,b; 1978a,b). They

Nucleoprotein structure of adenovirus

Creator:: Corden, Jeffry Lynn
Abstract:: The structure of the nucleoprotein core of adenovirus has been investigated. Using a combination of biophysical techniques and nuclease digestion I have determined that the adenovirus core, as isolated with pyridine, shares several physical properties with cellular chromatin. The most notable difference is the lack of a nucleosome repeat in...
Resource Type:: Dissertation

The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle

Creator:: Winzeler, Elizabeth A.
Abstract:: The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle were investigated by studying the time-resolved fluorescence anisotropy decays of ethidium bromide intercalated into the DNA of the core particle. As the torsional flexibility of DNA is affected by the presence of an intercalating...
Resource Type:: Masters Thesis
Full Text:: low salt transitions are sedimentation (Zama et al., 1978a; Gordon 3 et al., 1979), flow

ScholarsArchive@OSU

Active chromatin structure of Saccharomyces cerevisiae : high mobility group proteins, histone modifications and DNase I sensitivity

Hydrogen-stimulated CO₂ fixation and coordinate derepression of hydrogenase and ribulosebisphosphate carboxylase in a H₂-uptake positive strain of Rhizobium japonicum

Regulation of phosphofructokinase by reversible inactivation

Metabolic pathway of the nitrosoureas catalyzed by cytochrome P-450 and NADPH cytochrome P-450 reductase

The renaturation of bovine muscle pyruvate kinase

In vitro transcription in the yeast: Saccharomyces cerevisiae

Physical structure and reconstitution of chromatin core particles

Dependence of secondary structure of biopolymers on environment : a circular dichroism study of equivocal amino acid sequences in proteins and of left-handed DNA

Nucleoprotein structure of adenovirus

The effects of pH on the torsional flexibility of DNA bound to a nucleosome core particle

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