Index Catalog // ScholarsArchive@OSU

Genetics of germination-arrest factor (GAF) production by Pseudomonas fluorescens WH6: identification of a gene cluster essential for GAF biosynthesis

Creator:: Halgren, Anne, Maselko, Maciej, Azevedo, Mark, Mills, Dallice, Armstrong, Donald, and Banowetz, Gary
Abstract:: The genetic basis of the biosynthesis of the germination-arrest factor (GAF) produced by Pseudomonas fluorescens WH6, and previously identified as 4-formylaminooxyvinylglycine, has been investigated here. In addition to inhibiting the germination of a wide range of grassy weeds, GAF exhibits a selective antimicrobial activity against the bacterial plant pathogen Erwinia...
Resource Type:: Article

D-Lactate metabolism and the obligate requirement for CO2 during growth on nitrite by the facultative lithoautotroph Nitrobacter hamburgensis

Creator:: Starkenburg, Shawn R., Arp, Daniel J., and Bottomley, Peter J.
Abstract:: Nitrobacter hamburgensis X14 is a facultative lithoautotroph that conserves energy from the oxidation of nitrite (NO 2 ) and fixes carbon dioxide (CO2) as its sole source of carbon. The availability of the N. hamburgensis X14 genome sequence initiated a re-examination of its mixotrophic and organotrophic potential, as genes encoding...
Resource Type:: Article
Full Text:: untitled D-Lactate metabolism and the obligate requirement for

Thauera butanivorans sp. nov., a C₂–C₉ alkane-oxidizing bacterium previously referred to as ‘Pseudomonas butanovora’

Creator:: Dubbels, Bradley L., Sayavedra-Soto, Luis A., Bottomley, Peter J., and Arp, Daniel J.
Abstract:: The placement of ‘Pseudomonas butanovora’ in the genus Thauera was proposed previously, based on 16S rRNA gene sequence analysis, upon further studies of taxonomical characteristics. In this study, physiological characteristics and DNA–DNA reassociation data are presented and the transfer of ‘P. butanovora’ to the genus Thauera is proposed. The original...
Resource Type:: Article
Full Text:: -2902, USA 2Department of Microbiology, 220 Nash Hall, Oregon State University, Corvallis, OR 97331

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

Creator:: Cooley, Richard B., Dubbels, Bradley L., Sayavedra-Soto, Luis A., Bottomley, Peter J., and Arp, Daniel J.
Abstract:: Soluble butane monooxygenase (sBMO), a three-component di-iron monooxygenase complex expressed by the C2–C9 alkane-utilizing bacterium Thauera butanivorans, was kinetically characterized by measuring substrate specificities for C1–C5 alkanes and product inhibition profiles. sBMO has high sequence homology with soluble methane monooxygenase (sMMO) and shares a similar substrate range, including gaseous and...
Resource Type:: Article
Full Text:: 97331, USA 3Department of Microbiology, Oregon State University, Corvallis, OR 97331, USA Received 9

pA506, a Conjugative Plasmid of the Plant Epiphyte Pseudomonas fluorescens A506

Creator:: Stockwell, Virginia O., Davis, Edward W., II, Carey, Alyssa, Shaffer, Brenda T., Mavrodi, Dmitri V., Hassan, Karl A., Hockett, Kevin, Thomashow, Linda S., Paulsen, Ian T., and Lopera, Joyce E.
Abstract:: Conjugative plasmids are known to facilitate the acquisition and dispersal of genes contributing to the fitness of Pseudomonas spp. Here, we report the characterization of pA506, the 57-kb conjugative plasmid of Pseudomonas fluorescens A506, a plant epiphyte used in the United States for the biological control of fire blight disease...
Resource Type:: Article
Full Text:: -13. Copyright © 2013, American Society for Microbiology. All Rights Reserved. doi:10.1128/AEM.01354

Effect of Tannic Acid on the Transcriptome of the Soil Bacterium Pseudomonas protegens Pf-5

Creator:: Lim, Chee Kent, Penesyan, Anahit, Hassan, Karl A., Loper, Joyce E., and Paulsen, Ian T.
Abstract:: Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, sulfur metabolism, biofilm formation, motility, and...
Resource Type:: Article
Full Text:: article may be found at http://dx.doi.org/10.1128 /AEM.03101-12. Copyright © 2013, American Society for

Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5

Creator:: Hartney, Sierra L., Mazurier, Sylvie, Girard, Maeva K., Mehnaz, Samina, Davis, Edward W. II, Gross, Harald, Lemanceau, Philippe, and Loper, Joyce E.
Abstract:: The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric...
Resource Type:: Article
Full Text:: .01639-12. Copyright © 2013, American Society for Microbiology. All Rights Reserved. doi:10.1128/JB

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Creator:: Kurth, Elizabeth G., Peremyslov, Valera V., Prokhnevsky, Alexey I., Kasschau, Kristin D., Miller, Marilyn, Carrington, James C., and Dolja, Valerian V.
Abstract:: The improvement of the agricultural and wine-making qualities of the grapevine (Vitis vinifera) is hampered by adherence to traditional varieties, the recalcitrance of this plant to genetic modifications, and public resistance to genetically modified organism (GMO) technologies. To address these challenges, we developed an RNA virus-based vector for the introduction...
Resource Type:: Article

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Creator:: Carrington, James C., Freed, Deon D., and Sanders, Tatia C.
Abstract:: The virus-encoded proteins of tobacco etch virus (TEV), a plant potyvirus, arise by proteolytic processing of a large polyprotein precursor. The TEV genome codes for two proteinases, a 49-kilodalton proteinase and helper component proteinase (HC-Pro), which cleave the polyprotein at specific sites. The only known cleavage event catalyzed by HC-Pro...
Resource Type:: Article

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

Creator:: Schadd, Mary C., Haldeman-Chahill, Ruth, Cronin, Stephen, and Carrington, James C.
Abstract:: A mutational analysis was conducted to investigate the functions of the tobacco etch potyvirus VPgproteinase (NIa) protein in vivo. The NIa N-terminal domain contains the VPg attachment site, whereas the C-terminal domain contains a picornavirus 3C-like proteinase. Cleavage at an internal site separating the two domains occurs in a subset...
Resource Type:: Article
Full Text:: /96/$04.0010 Copyright q 1996, American Society for Microbiology Analysis of the VPg-Proteinase (NIa

ScholarsArchive@OSU

Genetics of germination-arrest factor (GAF) production by Pseudomonas fluorescens WH6: identification of a gene cluster essential for GAF biosynthesis

D-Lactate metabolism and the obligate requirement for CO2 during growth on nitrite by the facultative lithoautotroph Nitrobacter hamburgensis

Thauera butanivorans sp. nov., a C₂–C₉ alkane-oxidizing bacterium previously referred to as ‘Pseudomonas butanovora’

Kinetic characterization of the soluble butane monooxygenase from Thauera butanivorans, formerly ‘Pseudomonas butanovora’

pA506, a Conjugative Plasmid of the Plant Epiphyte Pseudomonas fluorescens A506

Effect of Tannic Acid on the Transcriptome of the Soil Bacterium Pseudomonas protegens Pf-5

Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5

Virus-Derived Gene Expression and RNA Interference Vector for Grapevine

Autocatalytic Processing of the Potyvirus Helper Component Proteinase in Escherichia coli and In Vitro

Analysis of the VPg-Proteinase (NIa) Encoded by Tobacco Etch Potyvirus: Effects of Mutations on Subcellular Transport, Proteolytic Processing, and Genome Amplification

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