Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
Freeze-drying, or lyophilization, has shown great promise in addressing many of the logistical challenges of storing and preserving red blood cells (RBCs). A crucial part of any RBC lyophilization protocol is the primary drying temperature, which affects the sample drying rate and the dried cake’s ability to form a stable...
Ice-free cryopreservation, known as vitrification, is an appealing approach for banking of adherent cells and tissues because it prevents dissociation and morphological damage that may result from ice crystal formation. However, current vitrification methods are often limited by the cytotoxicity of the concentrated cryoprotective agent (CPA) solutions that are required...
Cryopreservation of human red blood cells (RBCs) in the presence of 40% glycerol
allows a shelf-life of 10 years, as opposed to only 6 weeks for refrigerated
RBCs. Nonetheless, cryopreserved blood is rarely used in clinical therapy, in part
because of the requirement for a time-consuming (~1 h) post-thaw wash...
Effective methods for long-term preservation of cord red blood cells (RBCs) are needed to ensure a readily available supply of RBCs to treat fetal and neonatal anemia. Cryopreservation is a potential long-term storage strategy for maintaining the quality of cord RBCs for the use in intrauterine and neonatal transfusion. However,...
Loading of cryoprotectants into oocytes is an important step of the cryopreservation process, in
which the cells are exposed to potentially damaging osmotic stresses and chemical toxicity.
Thus, we investigated the use of physics-based mathematical optimization to guide design of
cryoprotectant loading methods for mouse and human oocytes. We first...
Granulocytes are currently transfused as soon as possible after collection because they rapidly deteriorate after being removed from the body. This short shelf life complicates the logistics of granulocyte collection, banking and safety testing. Cryopreservation has the potential to significantly increase shelf life; however, cryopreservation of granulocytes has proven to...
BACKGROUND: Simple and effective cryopreservation of human oocytes would have
an enormous impact on the financial and ethical constraints of human assisted
reproduction. Recently, studies have demonstrated the potential for cryopreservation
in an ice-free glassy state by equilibrating oocytes with high concentrations of
cryoprotectants (CPAs) and rapidly cooling to liquid...
Vitrification is a promising approach for cryopreservation of adherent cells because it allows complete avoidance of ice formation. However, high cryoprotectant (CPA) concentrations are required to prevent freezing, and exposure to high CPA concentrations increases the risk of osmotic and toxic damage. Although cell membrane transport modeling can be used...