Real-time PCR assays using locked nucleic acid (LNA) probes and high resolution melt (HRM) analysis were developed for molecular differentiation of Pseudoperonospora cubensis and P. humuli, causal agents of cucurbit and hop downy mildew, respectively. The assays were based on a previously identified single nucleotide polymorphism (SNP) in the cytochrome...
Groundnut ringspot virus (GRSV) and Tomato chlorotic spot virus
(TCSV) are two emerging tospoviruses in Florida. In a survey of the
southeastern United States, GRSV and TCSV were frequently detected in
solanaceous crops and weeds with tospovirus-like symptoms in south
Florida, and occurred sympatrically with Tomato spotted wilt virus (TSWV)...