Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...
An enzyme catalyzing the formation of
O-glucosylzeatin in immature embryos of P. lunatus was
purified 2500 fold using ammonium sulfate precipitation
followed by affinity and anion exchange chromatography.
The enzyme uses trans-zeatin as substrate (Km 28 uM) but
not cis-zeatin. ribosylzeatin, or dihydrozeatin. Both
UDP-glucose and UDP-xylose can serve as...
Sorbitol-induced osmotic stress (SIOS) to the root was found to be effective in
reducing transpirational water loss in plants during water stress. Mechanisms of SIOS in
stomatal control were investigated in containerized apples (Malus domestica Borkh.
'Gala'/M26) when leaf water potential (Ψw), osmotic potential (Ψs), turgor potential (Ψp),
stomatal conductance...
This research has focussed upon both the in vitro and in vivo characterization
of the recycling pathway leading from 5'-deoxy-5'-methylthioadenosine
to methionine in fruit. In addition to contributing towards polyamine
biosynthesis and other important cellular functions, in plant tissues
these events also serve to generate the hormone ethylene. A broad...
Cytokinins are a group of plant hormones mediating cell division and
differentiation. Zeatin is a naturally occurring and highly active cytokinin that is rapidly
metabolized in Phaseolus seeds. The metabolites and enzymes mediating the conversion
have been studied extensively. Zeatin metabolic enzymes may be utilized in studying the
regulation of...
The continuum theory provides a framework in which the growth
of a plant root as a dynamic process involving interactions among
transport of water and solute, cell division, and the subsequent cell
elongation can be described. A plant root is modeled as a one-dimensional,
multi-phase, mathematical continuum. The network of...