An ecological study of a microorganism similar to that isolated
in 1956 and designated as Micrococcus radiodurans indicated
that the organism could be isolated from several different environments.
By taking advantage of its radio-resistivity, high levels of
gamma radiation were used in the isolation procedures to inactivate
most of the...
Comparatively little work has been done on bacterial lipids.
There are profound differences between bacterial lipids in general
and lipids of the higher forms of life in such respects as the absence
of sterols, phospholipids low in nitrogen but high in inositol
and carbohydrate, the presence of large proportions of...
Studies were conducted under controlled conditions in order
to observe the resulting survival patterns in solid crabmeat of
Salmonella typhosa, Salmonella paratyphi B, and SaImonella wichita
following individual exposure to various doses of gamma radiation.
As shown in their survival curves, these species of Salmonella
exhibited in varying degrees a...
The purpose of this study was to compare and test the efficiency of the leading kinds of media being used for the isolation and identification
of Clostridium botulinum type E strains, and to develop a
more reliable method and media for the differentiation of this organism
based on the "pearly...
Routine studies of bacterial fatty acid and lipid composition,
made possible by recently developed techniques, have brought about
an increasing interest in the physiological significance of these
compounds. The purpose of this study was to characterize the fatty
acids of the vegetative cells of Clostridium botulinum 33A, as the
first...
Polyacrylamide gel disc electrophoresis of concentrated culture
media which had supported growth of Clostridium botulinum
types A and B was shown to be effective in separating the toxin
molecules from hemagglutinating molecules. Since the hemagglutinins
of the two types are immunologically identical and are present
in toxin and toxoid preparations,...
The primary purpose of this investigation was to characterize
the lipids of the spores and vegetative cells of Cl. botulinum. A
second purpose was to explore the possibility that lipids might serve
as a means of differentiating the chiefly proteolytic Cl. botulinum
type B from the nonproteolytic Cl. botulinum types...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...
Quantity variations in pigments and lipid classes were determined
following γ-irradiation of Micrococcus radiodurans, Sarcina
lutea, and Pseudomonas aeruginosa.
Pigmentation was determined by extraction with organic solvents
and measured as optical density at 475 mμ for M. radiodurans
and S. lutea, and 410 mμ for Ps. E aetuginosa. Pigments of...