Bdellovibrio bacteriovorus 100 and B. stolpii UKi-2 each produce
two extracellular proteases which attack Azocoll (collagen)
while B. starrii A3.12 produces at least four such proteolytic
enzymes. Under standard growth and assay conditions each Bdellovibrio
species produces a unique amount of proteolytic activity. The
decreasing order of enzyme units was...
The mechanisms of substrate recognition by regulatory proteases are not well understood. Presently, two opposing models have arisen to describe E. coil Lon's ability to discriminate between substrates: one suggests the N-terminus involvement while the second suggests the C-terminus involvement. In this project, the role of the C-terminal domain as...
Cathepsin B was the most active cysteine proteinase in the Pacific whiting
(Merluccius productus) fish fillet, and cathepsin L in surimi when the activities of the
most active cysteine proteinases (cathepsin L, B, and H) were compared. Cathepsin L
showed maximum activity at 55°C in both fish fillet and surimi,...
Surimi is a seafood product which is used to manufacture restructured products
such as artificial crab and lobster. Surimi is produced from fish fillets by washing to
remove sarcoplasmic proteins and increase the concentration of myofibrillar proteins, and
mixing with cryoprotectants. A valuable attribute of surimi is its ability to...
Kinetic properties of the two proteases, causing textural degradation of Pacific
whiting (Merluccius productus) during heating, were compared and characterized with the
synthetic substrate, Z-Phe-Arg-NMec. Pacific whiting P-I and P-II showed the highest
specificity on Z-Phe-Arg-NMec, specific substrate for cathepsin L. The K [subscript m] of
preactivated P-I and P-II...