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Some potential mechanisms for finely-tuned regulation of phospholipase C-β isozymes : studies of dimerization and phosphatidylinositol 3,4,5-trisphosphate activation

Creator:

Zhang, Yong

Abstract:

Phospholipase C-β (PLC-β) isozymes are key effectors in G protein-coupled signaling pathways. Prior research suggested that some isoforms of PLC-β may exist and function as dimers, but little is known about dimerization of PLC-β. Data from coimmunoprecipitation assays of differentially-tagged PLC-β constructs and sizeexclusion chromatography of native PLC-β support homodimerization...

Resource Type:

Dissertation

Full Text:

further intracellular signal transduction pathways (Berridge, 1993). Both IP3 and DAG are well-known
Properties of phospholipase C-β-mediated signaling in H9c2 cardiac myoblasts

Creator:

Kwon, Sun Hyung

Abstract:

The mechanisms by which the activities of phospholipase C-β (PLC-β) enzymes are negatively regulated have not been well defined. We used cardiac-derived ratmyoblast H9c2 cells to investigate possible modes of regulation of PLC-β3 enzymes,the only endogenous β isoform abundantly found in these cells. The PLC-β3population in these cells was detected...

Resource Type:

Masters Thesis

Full Text:

variety of cellular events, e.g. signal transduction, lipid transport, membrane internalization