Search Constraints

Start Over
Filtering by: Creator Barbar, Elisar Remove constraint Creator: Barbar, Elisar License All rights reserved Remove constraint License: All rights reserved

Search Results

  • Viral Targeting of Host Hubs: Interactions between the Rabies Virus and LC8

    Creator:
    Jespersen, Nathan E.
    Abstract:
    As obligate intracellular parasites, viruses rely on host machinery for their own reproduction. Viruses are therefore required to interact with a wide variety of host proteins, despite limitations in viral genome size. The most parsimonious method of success is to hijack central and essential proteins, known as hub proteins. LC8...
    Resource Type:
    Dissertation
    Full Text:
    Abstract approved: ______________________________________________________ Elisar J. Barbar As

  • Specificity, Allostery, and Multivalency in Binding to the Hub Protein LC8

    Creator:
    Estelle, Aidan B.
    Abstract:
    Interactions between proteins are essential to life, driving and regulating a majority of processes within all living cells. Study of protein-protein interactions reveals that some proteins act as hubs within networks of interactions, binding to many partner proteins. These hubs therefore are of particular importance to understanding protein function, interwoven...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Elisar Barbar Interactions between proteins are essential to life, driving and

  • Protein Disorder in the Evolution of Dynein Regulation

    Creator:
    Jie, Jing
    Abstract:
    Intrinsically disordered proteins (IDP) are a class of proteins that lack a three-dimensional structure and their prevalence and diverse functions in the cell have only been discovered relatively recently. The intermediate chain (IC) subunit of the microtubule motor protein complex dynein contains an N-terminal disordered region, N-IC, which is central...
    Resource Type:
    Dissertation
    Full Text:
    : _____________________________________________________ Elisar J. Barbar Intrinsically disordered proteins (IDP) are a class of proteins that lack

  • Context matters : assembly and disorder in the regulation of multivalent complexes

    Creator:
    Jara, Kayla A.
    Abstract:
    Intrinsically disordered proteins (IDPs), protein regions (IDRs), and protein complexes continue to emerge at the forefront of protein science. Proteins and protein regions lacking specific structure are found in all organisms, and often have vital roles in numerous biological processes. Breaking the well-known structure-function paradigm, the understanding of disorder-based functionality...
    Resource Type:
    Dissertation
    Full Text:
    . Abstract approved: ______________________________________________________ Elisar J. Barbar

  • Biophysical basis of macromolecular assembly in the dynein cargo attachment complex

    Creator:
    Hall, Justin Daniel
    Abstract:
    Cytoplasmic dynein is an ATP-dependent, microtubule-based molecular motor involved in the positioning and trafficking of cellular cargo. The cargo binding sub-domain of dynein contains the natively disordered intermediate chain (IC) and the homodimeric light chains (Tctex1, LC8 and LC7). The structure and stoichiometry of this complex, how the light chains...
    Resource Type:
    Dissertation
    Full Text:
    Attachment Complex . Abstract approved: Elisar J. Barbar

  • Three Sites are Better than One : Exploring Multivalency in LC8 Binding Partners

    Creator:
    Clark, Sarah A.
    Abstract:
    Hub proteins bind a large number of partners to facilitate structural changes and downstream protein interactions. LC8, a highly conserved protein homodimer, is a unique hub that regulates the activity of proteins in a wide range of cellular processes by binding to intrinsically disordered regions. With many of these systems,...
    Resource Type:
    Dissertation
    Full Text:
    : ______________________________________________________ Elisar J. Barbar Hub proteins bind a large number of partners to facilitate structural

  • Characterization of the Structure and Dynamics of the Intrinsically Disordered Dynein Intermediate Chain and its Interactions with Regulatory Binding Partners

    Creator:
    Morgan, Jessica L.
    Abstract:
    Cells represent microcosms of spatial and temporal structural organization, with the achievement of internal spatial organization relying upon a collection of macromolecular motor complexes to transport and localize components throughout the cell. Cytoplasmic dynein is one such motor complex, and is the principal ATP-dependent motor for retrograde transport along microtubules...
    Resource Type:
    Dissertation
    Full Text:
    ACKNOWLEDGEMENTS I thank my research supervisor, Elisar Barbar, particularly for her help with my application

  • Proteins in Motion: Protein Dynamics of Cataracts, and Interactions Between the COVID-19 Virus and its RNA Genome

    Creator:
    Masson-Forsythe, Heather M.
    Abstract:
    Just as the structure of a protein determines its function, protein motions and lack-of-structure are also key to regulating protein behavior. The human eye lens is made up of high concentrations of extremely stable crystallin proteins, but even in this context, small motions over time can cause a loss of...
    Resource Type:
    Dissertation

  • Determining cytokine induced vascular maturity throughimmunohistochemical double-staining

    Creator:
    Hosack, Luke William
    Abstract:
    As a major natural component of the extracellular matrix (ECM), hyaluronic acid (HA) is an excellent choice for biomimetic, biocompatible therapeutic materials. Furthermore, thiol-modified forms of HA are capable of forming macroporous hydrogels that allow for both controlled cytokine release and extensive vessel in-growth. It has previously been shown that...
    Resource Type:
    Masters Thesis

  • Biophysical analyses of peroxiredoxins and a partner reductase

    Creator:
    Hall, Andrea Renée
    Abstract:
    Peroxiredoxins (Prxs) are dominant peroxide-reducing enzymes with two important roles: they protect all organisms from oxidative damage induced by peroxides, and in eukaryotes, they participate in hydrogen peroxide signaling pathways. This dissertation presents studies aimed at the biophysical characterization of select Prxs and a Prx reductase to elucidate their structure-function...
    Resource Type:
    Dissertation
    Full Text:
    personally and scientifically, thank you. I thank my graduate committee members, Elisar Barbar, Janine