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  • Genomic-assisted determination of the natural nutrient requirements of the cosmopolitan marine bacterium 'Candidatus Pelagibacter ubique'

    Creator:
    Tripp, H. James
    Abstract:
    To identify factors limiting 'Candidatus Pelagibacter ubique' maximum cell density and growth rate in pure culture on seawater, the genome sequence of 'Cand. P. ubique' was analyzed, culturing experiments with organic and inorganic nutrient additions were made, and radiotracer uptake experiments were performed. The genome was sequenced, custom data mining...
    Resource Type:
    Dissertation
    Full Text:
    -1. Sulphur metabolism pathways and comparative genomics.. ............................... 101 6

  • Cloning and characterization of GRASP, a novel retinoic acid-induced gene from P19 embryonal carcinoma cells

    Creator:
    Nevrivy, Daniel
    Abstract:
    Retinoic acid (RA) exerts important effects in the processes of vertebrate development, cellular growth and differentiation, and homeostasis. However, the mechanisms of action of RA in the control of cellular and developmental processes are incompletely understood, as the retinoid target genes have not been fully characterized. The goal of these...
    Resource Type:
    Dissertation
    Full Text:
    , transport, metabolism, and utilization of retinol and its derivatives requires the actions of a diverse

  • Characterization of rodent selenoprotein W promoter

    Creator:
    Amantana, Adams
    Abstract:
    Rat selenoprotein W (SeW) promoter activity was investigated using different concentrations of cadmium, copper, and zinc. Two fragments (404bp and 1265bp) of the SeW promoter, containing a single metal response element (MRE), were ligated into the multiple cloning site of a pGL3-Basic reporter plasmid. The constructs were transfected into cultured...
    Resource Type:
    Dissertation
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    Dr. William Vorachek and Mrs. Judy Butler for their immense assistance in every aspect of the

  • Transcriptional networks controlled by the fatty acid regulators FAR-1 and FAR-2

    Creator:
    Bredeweg, Erin L.
    Abstract:
    Fungi are capable of growth on a wide variety of carbon sources, both living and dead. They can produce an arsenal of enzymes and transporters for harvesting sugars, polysaccharides, amino acids, lipids and micronutrients from their environments [1]. Within the nucleus of a cell, transcription factors (TF) control whether genes...
    Resource Type:
    Dissertation
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    ! metabolism,!oxidative!stress!response,!and!development.! Transcription!factor!networks!consist!of!describing

  • Metabolism of one carbon (C1) and methylated compounds in SAR11 bacteria

    Creator:
    Sun, Jing
    Abstract:
    SAR11 Alphaproteobacteria are the most abundant aerobic chemoheterotrophs in ocean surface waters. Previous studies have indicated SAR11 cells play an important role in marine carbon cycling and consume up to half of some common dissolved organic compounds, such as amino acids. During sequencing of the first SAR11 genome, genes for...
    Resource Type:
    Dissertation
    Full Text:
    genetic, biochemical and physiological studies of C1 metabolism (Chistoserdova et al., 2005). 1.5.1.1

  • Metabolic engineering of cyanobacteria to optimize photobiological hydrogen production

    Creator:
    Luterra, Markael
    Abstract:
    Photobiological hydrogen production -- the use of photosynthesis to split water into hydrogen and oxygen -- presents an opportunity for efficient and large-scale solar biofuel development. One of the major challenges in this area is competition for reductant between the hydrogen-evolving hydrogenase enzyme and other metabolic pathways. In this work...
    Resource Type:
    Dissertation
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    hydrogen production. These pathways are logical targets for genetic manipulation with the goal of

  • SLC23A1, the gene encoding sodium-dependent vitamin C transport protein 1 (SVCT1) : regulation of transcription and its functional consequences

    Creator:
    Michels, Alexander Johannes
    Abstract:
    Vitamin C is an essential component of the human diet. Uptake of vitamin C in the cell is regulated by the function of sodium-dependent vitamin C transporters (SVCTs). SVCT1 (encoded by the SLC23A1 gene) is expressed in the colon, liver and kidney, where it plays a vital role in determining...
    Resource Type:
    Dissertation
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    genetic regulation. We isolated a 1239-base-pair fragment of the SLC23A1 5’-flanking sequence from

  • Model Predictive Control for Optimum Algal Growth

    Creator:
    Juneja, Ankita
    Abstract:
    Microalgae has been under careful consideration as a sustainable feedstock for renewable fuel recently due to its year round production with high energy yields per unit area, reduced need for arable land, water treatment benefits with nutrient cycling, and minimal competition with the food industry. Despite of all these advantages,...
    Resource Type:
    Dissertation
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    . .......................................................................................................... 83 3.4 Chitosan synthesis pathway added in amino sugar and nucleotide sugar metabolism pathway

  • Transcriptional regulation of mouse epidermal permeability barrier development and homeostasis by Ctip2

    Creator:
    Wang, Zhixing
    Abstract:
    Skin is the largest organ in the body that protects the organism from environmental, chemical and physical traumas of each passing day. The protective skin epidermal permeability barrier (EPB) is formed within the exterior layers of the epidermis, which are regularly sloughed off and repopulated by movement of inner cells....
    Resource Type:
    Dissertation
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    identified Ctip2 as a key regulator of skin lipid metabolism. Germline deletion of Ctip2 in mouse embryos