Environmental DNA sequencing has revealed the expansive biodiversity of microorganisms
and clarified the relationship between host-associated microbial communities and host
phenotype. Shotgun metagenomic DNA sequencing is a relatively new and powerful
environmental sequencing approach that provides insight into community biodiversity and
function. But, the analysis of metagenomic sequences is complicated...
Identifying the ecological and evolutionary mechanisms that determine biological diversity is a central question in ecology. In microbial ecology, phylogenetic diversity is an increasingly common and relevant means of quantifying community diversity, particularly given the challenges in defining unambiguous species units from environmental sequence data. We explore patterns of phylogenetic...
Growing evidence indicates that disrupting the microbial community that comprises the intestinal tract, known as the gut microbiome, can contribute to the development or severity of disease. As a result, it is important to discern the agents responsible for microbiome disruption. While animals are frequently exposed to a diverse array...
Many obligate symbiotic fungi are difficult to maintain in culture, and there is a growing need for alternative approaches to obtaining tissue and subsequent genomic assemblies from such species. In this study, the genome of Elaphomyces granulatus was sequenced from sporocarp tissue. The genome assembly remains on many contigs, but...
Introduction. Age is the primary risk factor for major human chronic diseases, including cardiovascular disorders, cancer, type 2 diabetes, and neurodegenerative diseases. Chronic, low-grade, systemic inflammation is associated with aging and the progression of immunosenescence. Immunosenescence may play an important role in the development of age-related chronic disease and the...
Shotgun metagenomic DNA sequencing is a widely applicable tool for characterizing the functions that are encoded by microbial communities. Several bioinformatic tools can be used to functionally annotate metagenomes, allowing researchers to draw inferences about the functional potential of the community and to identify putative functional biomarkers. However, little is...
BACKGROUND: Targeted capture of genomic regions reduces sequencing cost while generating higher coverage by
allowing biomedical researchers to focus on specific loci of interest, such as exons. Targeted capture also has the
potential to facilitate the generation of genomic data from DNA collected via saliva or buccal cells. DNA samples...
Thomas and Megahan [1998] provide a thoughtful reanalysis of our earlier paper [Jones and Grant, 1996]. Thomas and Megahan [1998] (hereinafter referred to as T&M) reacted to three assertions made by Jones and Grant [1996] (hereinafter referred to as J&G): (1) "forest harvesting has increased peak discharges by as much...
This study shows how patronage framed and fashioned the careers of Thomas Hobbes and
his patron William Cavendish, the earl of Newcastle. Newcastle's protection allowed
Hobbes to articulate heterodox ideas without immediate fear of reprisal. It also enabled
him to solidify his status as a gentleman and the intellectual equal...