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  • Biosynthesis and modification of the antibiotic enduracidin

    Creator:
    Goebel, Neal C.
    Abstract:
    The continued propagation of antibiotic resistance requires the development of new therapeutics. The lipopeptide antibiotic enduracidin has demonstrated high activity against Gram-positive pathogens including methicillin-resistant Staphylococcus aureus. In addition to a lack of cross-resistance with existing antibiotic classes, enduracidin has no known transferrable resistance mechanism. The development of enduracidin as...
    Resource Type:
    Dissertation

  • Activation, incorporation and modification of capreomycidine during viomycin biosynthesis

    Creator:
    Fei, Xiaobo
    Abstract:
    Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. The nonproteinogenic amino acid capreomycidine is the signature residue found in the tuberactinomycin family of antitubercular peptide antibiotics and an important element of the pharmacophore. Recombinant VioG, a single module peptide synthetase from the viomycin gene cluster cloned...
    Resource Type:
    Masters Thesis

  • Formation and modification of enduracididine, a nonproteinogenic amino acid

    Creator:
    Tan, Ying
    Abstract:
    Molecular genetic and enzymological techniques have been employed to study antibiotic biosynthesis. In this thesis, we studied the formation and modification of the nonproteinogenic amino acid enduracididine (End), which exists in two important antibiotics, mannopeptimycins (MPPs) and enduracidin. Sequence analysis of the MPP gene cluster revealed that the product of...
    Resource Type:
    Masters Thesis

  • Biosynthetic studies of fungal diterpene antibiotics

    Creator:
    Yao, Qingmei
    Abstract:
    The isoprenoid pathway is one of the major biosynthetic pathways of secondary metabolites. Isoprenoids with a C₂₀ skeleton are known as diterpenoids and are derived from the common precursor, geranylgeranyl diphosphate (GGPP). This dissertation describes approaches to discover the diterpene synthase enzymes which provide the backbone structures for pleuromutilin and...
    Resource Type:
    Masters Thesis

  • Biosynthetic engineering of new pactamycins

    Creator:
    Lu, Wanli
    Abstract:
    Among the myriad of naturally occurring bioactive compounds are the aminocyclopentitol-containing natural products that represent a family of sugar-derived microbial secondary metabolites, such as the antibiotics pactamycin, allosamidin, and trehazolin. Pactamycin, a structurally unique aminocyclitol antibiotic isolated from Streptomyces pactum, consists of a 5-membered ring aminocyclitol (cyclopentitol) unit, two aromatic...
    Resource Type:
    Dissertation