The production of C¹⁴O₂ metabolized from glucose labeled in
specific carbon atoms by homogenates of chick embryonic and adult
heart was compared by the radiorespirometric technique. Homogenates
of early (two to five days of incubation) embryo hearts were
found to utilize the phosphogluconate pathway of glucose catabolism
to a greater...
The catabolism of glucose by proliferating and resting cells of
two strains of Saccharomyces cerevisiae has been examined, with
respect to the nature and participation of concurrent glucose pathways
by means of the radiorespirometric method. The nicotinamide
nucleotide content, and nicotinamide nucleotide oxidase activities
in these cells were also examined....
The enzymes and pathways involved in the catabolism of
glucose by several strains of Streptococcus diacetilactis, Streptococcus
cremoris, and Streptococcus lactis, commonly called the
lactic streptococci, were studied. The presence of aldolase,
triosephosphate isomerase and alcohol dehydrogenase in these
organisms provided evidence for the operation of the Embden-Meyerhof-Parnas (EMP) pathway...
A photon-counting technique which provides direct digital read-out
of data has been developed and evaluated for the reaction rate determination
of glucose. The enzyme catalyzed oxidation of glucose
to gluconic acid and hydrogen peroxide with the subsequent reaction of
hydrogen peroxide with excess iodide to produce tri-iodide is followed
by...
The inhibitory effect of 2-deoxy-D-glucose (2-DG) upon the
metabolism of glucose and fructose by proliferating cells of
Saccharomyces cerevisiae (OSU strain 2) has been examined. By
the use of radiotracer methods, particularly radiorespirometric
techniques, it has been possible to demonstrate that the rate of
hexose assimilation by yeast cells is...
Existing methods for the estimation of relative participation of
glucose catabolic pathways make use of yield data of respiratory
¹⁴CO₂ or intermediary ¹⁴C products derived from glucose substrates
specifically labeled with ¹⁴C. Cumulative yields of respiratory
¹⁴CO₂ observed at the end of time course of complete substrate
utilization are analyzed...
Glucose metabolism of phage-infected Streptomyces griseus
was examined by using the specific radioactivities of the amino
acids from the extracted protein of the host and phage as an index
of the catabolic pathways of glucose metabolism. Conventional
methods for the study of phage-infected bacteria did not suit the
host-actinophage system...