Conversion of exogenous cis-zeatin to trans-zeatin in immature seeds of
Phaseolus vulgaris L. led to the isolation of a cis-trans isomerase from the endosperm.
The enzyme was purified over 2000-fold by chromatography on a series of FPLC
(anion exchange, gel filtration, and hydrophobic interaction) and Concanavalin A
columns. Non-enzymatic isomerization...
Cytokinin oxidase activity in callus tissues of
Phaseolus vulgaris L. cv. Great Northern has been examined
using an assay based on the oxidation of radioactively
labeled N⁶ -(Δ² -isopentenyl) adenine (i⁶Ade) to adenine
(Ade). Conditions for the quantitative extraction and
assay of the enzyme have been established. The substrate
specificity...