The primary purpose of this investigation was to characterize
the lipids of the spores and vegetative cells of Cl. botulinum. A
second purpose was to explore the possibility that lipids might serve
as a means of differentiating the chiefly proteolytic Cl. botulinum
type B from the nonproteolytic Cl. botulinum types...
Gamma irradiation inactivation kinetics were studied using
fifteen organisms naturally contaminating seafoods. Survival curves
for seven Salmonella, three Shigella, two Streptococcus species and
a Staphylococcus, Escherichia and Proteus species were determined
using Hartsell's broth and fresh oysters. Also, the Shigella species
and one species of Streptococcus, Escherichia and Proteus were...
The difficulties encountered in working with micro-quantities
of lipids have been overcome by the advent of new and improved
procedures in chromatography which have made possible better
fractionation and identification. Bacterial lipids because of their
different lipid pattern from other life forms have stimulated much
interest. Studies have been made...
Quantity variations in pigments and lipid classes were determined
following γ-irradiation of Micrococcus radiodurans, Sarcina
lutea, and Pseudomonas aeruginosa.
Pigmentation was determined by extraction with organic solvents
and measured as optical density at 475 mμ for M. radiodurans
and S. lutea, and 410 mμ for Ps. E aetuginosa. Pigments of...
The role of nematodes (Diplogaster lheritieri Maupas 1919) as
excretors of pathogenic organisms was studied. Salmonella typhi and
Salmonella wichita were fed to nematodes for two days. The nematodes
were surface sterilized with free chlorine. The activity of the
chlorine was stopped by the addition of sodium thiosulfate followed by...
Disc gel electrophoresis of the vegatative cell -free extracts ofstrains of Clostridium botulinum types A, B, C, E, and F and therelated nontoxic group showed limited value as a means for identifyingthese closely related microorganisms, since separation,though not consistent in all cases, could only be based on the numberof protein...
Several biochemical tests were studied to determine their
correlation with coagulase activity. The lysozyme test was in perfect
agreement with the coagulase test for the strains studied.
Mannitol fermentation and egg yolk lipase tests were in agreement
with the coagulase test for 96% of the coagulase positive strains.
Sheep red...
Concentrated culture supernatants from strains of Vibrio
parahaemolyticus of gastroenteric origin, "suspected" V. parahaemolyticus
isolated from cases of skin infection, nonpathogenic marine
Vibrio, V. anguillarum and V. alginolyticus, were submitted to flat
gel electrophoresis, followed by the examination of the polyacrylamide
gels for total protein and enzyme patterns.
Variations related...
The purpose of this study was to examine the culture
supernatants of various strains of Clostridium botulinum
for characteristic extracellular enzymes which can facilitate
their rapid identification.
A convenient, efficient, water-cooled flat gel electrophoretic
unit was utilized to concentrate and purify the
total proteins and extracellular enzymes present in the...