Batch sorption tests were conducted using 3,4-DCP and cellulose triacetate as the sorbate/sorbent pair. Sorbate concentrations
in the liquid were sampled with time and analyzed by electron
capture detector gas chromatography. Sorption was found to be a two
stage process characterized by a rapid initial uptake followed by a
extended...
Surface tension kinetics exhibited by selected stability mutants of T4 lysozyme at the air-water interface were monitored with DuNoüy tensiometry. Mutant lysozymes were produced by substitution of isoleucine at position 3 with cysteine, leucine, tryptophan and glycine. Each substitution resulted in an altered structural stability quantified by a change in...
The adsorption kinetics exhibited by selected charge mutants of T4 lysozyme at
silanized silica surfaces were monitored with in situ ellipsometry. Mutant lysozymes were
produced by substitution of lysine (Lys) with glutamic acid (Glu). Each substitution
resulted in a decrease in the net charge of the protein by 2 units....
In this research, the interfacial behavior of Trichoderma reesei CBHI and Thermomonospora fusca E₅ cellulases were studied at synthetic surfaces. For this purpose, colloidal silica and polystyrene particles were used to prepare cellulase-particle suspensions that were analyzed by several solution-phase techniques. These included circular dichroism spectroscopy, size exclusion chromatography and...