The binding of fifteen hydrocarbons to native DNA
has been investigated. The hydrocarbons were chosen to
test a size criterion for hydrocarbon - DNA complexes. It
has been found that hydrocarbons, which are small enough
to intercalate between the base pairs of a distorted Watson -
Crick model of DNA,...
Work is presented which explores the properties and nature of
the P-form of DNA. Initially the P-form represented an unknown
state of DNA which occurred when this molecule was in a solvent of
47.5% methanol, 5% water, and 47.5% ethanol, and which showed properties
of both native and denatured conformations....
An attempt was made to differentiate between runs of steelhead by comparing the reassociations of their DNA. Conditions for the reassociation were studied using E. coli DNA, and observations
were made spectrophotometrically. Of the five buffers examined, 1.0 molar sodium perchlorate plus one-hundredth molar tris-HCl, pH 7.1, at 60° or...
This dissertation describes an investigation of DNA precursor supply for mitochondrial and nuclear DNA synthesis in HeLa cells and Mouse L-cells. HeLa cells were used for the quantitation of cellular and mitochondrial deoxyribonucleoside triphosphate (dNTP) and ribonucleoside triphosphate (rNTP) pools and of changes in pools in response to treatment with...
A fluorescent dye, 1-dimethylaminonaphthalene-5-sulfonyl
chloride (dansyl chloride), was covalently bonded to polylysine. The
dansyl tagged polylysine was complexed to DNA by gradient dialysis.
The polarization and intensity of fluorescence of the dansyl and the
turbidity of the complex were measured as a function of salt concentration
for a variety of...
The genome composition and DNA base sequence homologies
of several diverse species of fish were examined. The fishes were
characterized from spectrophotometric studies of reassociation and
hybridization of their DNAs and from the thermal stabilities of hybrid
duplexes.
Spectrophotometric studies indicated that repeated sequences
comprise 35-40% of the genomes of...
Investigators have been studying an aggregate
containing enzymes of deoxyribonucleoside triphosphate
biosynthesis in T4-phage infected bacteria. They suggest
that it behaves as an organized complex, efficiently
channeling DNA precursors to the replication apparatus.
Previous work has suggested that about 10 enzyme
activities remain associated through several fractionation
steps. This multi-enzyme...
The interaction of polylysine with DNA may be divided into two
separate phenomena: the primary interaction involved in formation of
a well-defined complex, and secondary interactions leading to aggregation
of these complexes. Most studies on the interaction of polylysine
with DNA have used techniques which measure properties of
the aggregated...