Regulation of expression of three genes in the polyhedron
envelope protein (PEP) gene region of the Orgyia pseudotsugata
multinucleocapsid nuclear polyhedrosis virus (OpMNPV) was
examined. These genes include open reading frame (ORF) 1 (encoding
p21), ORF 2 (encoding gp16), and ORF 3 (encoding the polyhedron
envelope protein). The effect of...
The yeast two-hybrid system was used to examine
interactions between the nine proteins involved in baculovirus DNA
replication. From the six proteins required for DNA replication,
four protein-protein interactions were identified, including an
interaction between LEF-1 and LEF-2, LEF-3 and itself, LEF-3 and
P143 (Helicase), and an interaction between IE-1...
The Baculoviridae comprise a diverse group of occluded DNA viruses that contain large double-stranded DNA genomes of 80 - 180 kb and may encode up to 180 gene products. To understand how baculoviruses replicate and process their genomes and the gene products that are involved in these events, a series...
A capsid protein termed p87 of the Orgyia pseudotsugata multicapsid nuclear polyhedrosis virus (OpMNPV) was located to the HindIII-G fragment of the viral genome by Southern hybridization. A 3.6 kb segment of this region was sequenced and an 1872-nt open reading frame encoding the p 87 protein was identified and...