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  • Use of elicitor sets to characterize cellular signal transduction networks

    Creator:
    Narayanan, Arthi
    Abstract:
    Intracellular signaling cascades can no longer be viewed as linear pathways that relay and amplify information. Often, components of different pathways interact, resulting in signaling networks. The interactions of different pathways and the dynamic modulation of the activities of the components within signaling pathways can create a multitude of biological...
    Resource Type:
    Masters Thesis
    Full Text:
    Sets to Characterize Cellular Signal Transduction Networks Abstract AnDroved: Frank W.R. Chaplen

  • Some potential mechanisms for finely-tuned regulation of phospholipase C-β isozymes : studies of dimerization and phosphatidylinositol 3,4,5-trisphosphate activation

    Creator:
    Zhang, Yong
    Abstract:
    Phospholipase C-β (PLC-β) isozymes are key effectors in G protein-coupled signaling pathways. Prior research suggested that some isoforms of PLC-β may exist and function as dimers, but little is known about dimerization of PLC-β. Data from coimmunoprecipitation assays of differentially-tagged PLC-β constructs and sizeexclusion chromatography of native PLC-β support homodimerization...
    Resource Type:
    Dissertation
    Full Text:
    further intracellular signal transduction pathways (Berridge, 1993). Both IP3 and DAG are well-known

  • Regulation of phospholipase C-beta isozymes by calmodulin

    Creator:
    McCullar, Jennifer Star
    Abstract:
    Phospholipase C-Jβ (PLCβ) is an important effector enzyme in G-protein-coupled signaling pathways. Activation of PLCβ by Gα and Gβy subunits has been fairly well characterized, but little is known about other protein interactions that may also regulate PLCβ function. A yeast two-hybrid screen of a mouse brain cDNA library with...
    Resource Type:
    Dissertation
    Full Text:
    the degree of Doctor of Philosophy in Molecular and Cellular Biology presented on September 22. 2005

  • Analysis of putative elements of plant signal transduction chains

    Creator:
    Verhey, Steven D.
    Abstract:
    The thesis begins with an introduction to signal transduction and an analysis of current understanding of plant signal transduction. There are similarities between plants and animals, but also key differences, including lack of protein kinase C and of a cAMP signaling pathway in plants, and presence in plants of calcium...
    Resource Type:
    Dissertation
    Full Text:
    Analysis of putative elements of plant signal transduction

  • A two-component regulatory system controlling antibiotic production by Pseudomonas flourescens Pf-5

    Creator:
    Corbell, Nathan
    Abstract:
    Tn5 mutagenesis of apdA (for antibiotic production) and deletion of gacA (for global antibiotic and cyanide) resulted in the same pleiotropic phenotype in Pseudomonas fluorescens (i.e. production of an array of secondary metabolites including the antibiotics pyrrolnitrin, pyoluteorin, and 2,4-diacetylphloroglucinol as well as a tryptophan-side-chain oxidase, hydrogen cyanide, and an...
    Resource Type:
    Dissertation
    Full Text:
    sense and respond to their environment. Two-component systems regulate such diverse cellular processes

  • In vitro and in vivo approaches for the functional characterization of the scaffold protein, GRASP

    Creator:
    Venkataraman, Anand
    Abstract:
    Studies using the pluripotent embryonic carcinoma cell line, P19, as a retinoic acid (RA)-responsive model system have been instrumental towards our understanding of the RA-dependent signaling pathways in development and homeostasis. Grp1-associated scaffold protein (GRASP; also known as Tamalin) was first identified by our group, as a gene robustly induced...
    Resource Type:
    Dissertation
    Full Text:
    Molecular and Cellular Biology presented on November 10, 2009. Title: In vitro and In vivo

  • Properties of phospholipase C-β-mediated signaling in H9c2 cardiac myoblasts

    Creator:
    Kwon, Sun Hyung
    Abstract:
    The mechanisms by which the activities of phospholipase C-β (PLC-β) enzymes are negatively regulated have not been well defined. We used cardiac-derived ratmyoblast H9c2 cells to investigate possible modes of regulation of PLC-β3 enzymes,the only endogenous β isoform abundantly found in these cells. The PLC-β3population in these cells was detected...
    Resource Type:
    Masters Thesis
    Full Text:
    variety of cellular events, e.g. signal transduction, lipid transport, membrane internalization

  • A network approach for the mechanistic classification of bioactive compounds

    Creator:
    Siebert, Trina A.
    Abstract:
    Using network architecture to describe a biological system is an effective organizational method. The utility of this approach, which generally applies to qualitative models, is enhanced by the addition of quantitative models characterizing the interactions between network nodes. A chromatophore-based signal transduction network is developed, and the highly interconnected major...
    Resource Type:
    Dissertation
    Full Text:
    CLASSIFICATION OF BIOACTIVE COMPOUNDS USING A CELLULAR SIGNAL TRANSDUCTION NETWORK..................... 8 2.1

  • Investigating the ecological role of cell signaling in free-living marine heterotrophic protists

    Creator:
    Hartz, Aaron J.
    Abstract:
    Phagotrophic protists are major consumers of microbial biomass in aquatic ecosystems. However, biochemical mechanisms underlying prey recognition and phagocytosis by protists are not well understood. We investigated the potential roles of cell signaling mechanisms in chemosensory response to prey, and in capture of prey cells, by a marine ciliate (Uronema...
    Resource Type:
    Masters Thesis
    Full Text:
    
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