We isolated a methanogen from deep in the sediments of the Nankai Trough off the eastern coast of Japan.
At the sampling site, the water was 950 m deep and the sediment core was collected at 247 m below the sediment
surface. The isolated methanogen was named Nankai-1. Cells of...
PCR amplification, restriction fragment length polymorphism, and phylogenetic analysis of oxygenase genes
were used for the characterization of in situ methane- and ammonia-oxidizing bacteria from free-living and
attached communities in the Eastern Snake River Plain aquifer. The following three methane monooxygenase
(MMO) PCR primer sets were used: A189-A682, which amplifies...
Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell numbers generally decreased with sediment...
The bacteria colonizing geologic core sections (attached) were contrasted with those found suspended in the groundwater (unattached) by examining the microbiology of 16 depth-paired core and groundwater samples using a suite of culture-independent and culture-dependent analyses. One hundred twenty-two meters was continuously cored from a buried chalcopyrite ore hosted in...
Changes in climate caused by increased concentrations of carbon dioxide (CO₂) in the Earth’s atmosphere have led land and ocean surface temperatures to increase by 0.85°C and sea level to increase by 19 cm relative to preindustrial times. Global climate change will lead to further alterations in mean temperature and...
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Mark E. Harmon
Changes in climate caused by increased concentrations of carbon dioxide (CO2
We present a new nitrogen isotope model incorporated into the three-dimensional ocean component of a global Earth system climate model designed for millennial timescale simulations. The model includes prognostic tracers for the two stable nitrogen isotopes, 14N and 15N, in the nitrate (NO3−), phytoplankton, zooplankton, and detritus variables of the...
Global plant genetics research efforts have focused on developing high yielding, stress tolerant, and disease resistant row and field crop varieties that are more efficient in their use of agronomic inputs (water, nutrients, pesticides, etc.). Until recently, a key bottleneck in such research was the lack of high-throughput sensing technologies...
Kernel hardness (KHA) is a major factor determining break flour yield (BFY) and end-use quality of common wheat (Triticum aestivum L.). Within the soft wheat class, genotypes with consistently softer grains than common soft wheat are considered to be 'extra-soft'. In addition, 'extra-soft' wheats have greater BFY than common soft...
The degradation of organic carbon in subseafloor sediments on continental margins contributes to the largest reservoir of methane on Earth. Sediments in the Andaman Sea are composed of ~ 1% marine-derived organic carbon and biogenic methane is present. Our objective was to determine microbial abundance and diversity in sediments that...