An investigation was undertaken to determine whether
potassium is indeed necessary for the in vivo synthesis of a
specific protein. The effects of univalent cations on the
inductive formation of nitrate reductase and on the level of
activity of representative non-adaptive enzyme are presented.
Cultures of Neurospora crassa were grown...
Experiments are reported which demonstrate that a cobalt deficiency
in R. meliloti results in a decreased cytochrome content of
bacterial cells. It is concluded that the effect of cobalt deficiency
on cytochrome content of Rhizobium cells and on the leghemoglobin
content of nodules possibly may be explained by an effect...
Ribonucleotide reductase from Rhizobium meliloti has been
partially purified and characterized. The enzymatic reduction of
ribonucleotides to deoxyribonucleotides is dependent upon B₁₂ coenzyme
and dihydrolipoate.
B₁₂ coenzyme was more effective than B₁₂ coenzyme analogues
in the reductase reaction. The affinity of the R. meliloti
reductase system for B₁₂ coenzyme was...
Alnus rubra (Bong. ) seedlings were grown in sand culture and
irrigated with nutrient solution containing CdCl₂
ranging from 5 μg to 100 mg per liter. Treatment of A. rubra seedlings for 4 weeks
with 50 and 100 mg CdCl₂
per liter of nitrogen-free nutrient solution
decreased in situ nitrogenase...
A major objective of this investigation was to determine the
possible roles, if any, of poly-β-hydroxybutyrate and products of
its metabolic breakdown in symbiotic nitrogen fixation by nodule
bacteroids. Changes in poly -β- hydroxybutyrate content and in
activities of nitrogenase, β-hydroxybutyrate dehydrogenase, and
isocitrate lyase were studied under various conditions...
The primary purpose of this investigation was to determine the
conditions necessary for the preparation of cell-free extracts of
nodule bacteroids capable of catalysing the reduction of acetylene to
ethylene and nitrogen to ammonia. Initial investigations were conducted
to determine whether intact soybean root nodules would reduce
acetylene to ethylene...