Cell migration is fundamental for physiological processes such as immune re-sponses, wound healing and development. It is also a critical characteristic of metastatic cancer cells. An extensive amount of studies have focused on 2D sub-strates in order to understand the mechanism of cancer metastasis. However, much less is known about...
Cancer is a hard disease to control because of its ability to spread to different parts of the body. Cancer cells can spread out from a primary tumor to distant organs which gives rise to new cancer cell colonies and more tumors over time. This process is known as metastasis...
Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed...
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Three-Dimensional Reflectance Traction
Microscopy
JihanKim, Christopher A. R. Jones, Nicholas
Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed...
Cells in three-dimensional (3D) environments exhibit very different biochemical and biophysical phenotypes compared to the behavior of cells in two-dimensional (2D) environments. As an important biomechanical measurement, 2D traction force microscopy can not be directly extended into 3D cases. In order to quantitatively characterize the contraction field, we have developed...
Type I collagen fibers, the connective tissue that makes up the human breast, can be easily manipulated by cells or other outside factors. In previous studies, the alignment of collagen has been associated with the beginning of metastatic cancer, possibly influencing cancerous cells to migrate from the tumor. In this...
Current methods in traction force analysis rely on a homogeneous extra-cellular matrix and
embedded microbeads to track deformations. The efficacy of these methods decreases when the matrix is not homogeneous. In heterogeneous matrices, beads often aggregate in regions of higher matrix density and float in regions where matrix substrate is...
Cancer cell migration in three-dimensional extracellular matrix is a major cause of death for cancer patients. Although extensive studies have elucidated detailed mechanism of single cell 3D invasion and cell-ECM interaction, 3D collective cancer invasion is still poorly understood. 3D collective migration models have unveiled unexpected degrees of diversity and...
The utility of high-throughput, computational screening has become an invaluable asset to the field of materials science. In the hierarchy of computational methods, the most accurate methods are often the most computationally expensive. However, as both the efficiency and fidelity of numerical techniques advance, high-quality screening of large materials datasets...
Deep learning has recently revolutionized robot perception in many canonical robotic applications, such as autonomous driving. However, a similar transformation has yet to occur in more harsh environments including underwater and underground. This is due in part to the difficulty in deploying robots in these environments, which lack large real...
