Cuphea laminuligera Koehne and Cuphea lutea Rose in Koehne are
undomesticated species having potential as oilseed crops. Genetic
markers and mating systems have not been described for these
species. Our objectives were to survey these species for allozyme
variation, analyze the segregation and linkage of polymorphic loci,
estimate autofertility and...
We are developing a flavin-containing (Fmo) knock-out mouse for Fmo2 that will be utilized to elucidate the role of active human FMO isoform 2 (FMO2.1) in xenobiotic metabolism and toxicity. Since FMO2 is primarily expressed in lung this will provide information on how 27% of African-Americans and 5% of Hispanics...
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Full Text:
. SharonKrueger
• Professor David Williams
• Lindsey Shorey
Williams Lab
• Center for Genome Research
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Full Text:
with environmental PAH mixtures
Andrew Larkina,b, Lisbeth K. Siddensb, Sharon K. Kruegerc, David E
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
Full Text:
Hydrogen Peroxide
Siddens, L. K., Krueger, S. K., Henderson, M. C., & Williams, D. E. (2014).
Mammalian
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin...
