Isocitrate-NADP oxidoreductase activity in mitochondrial and
soluble fractions was determined for hearts, livers and brains of
chick embryos at various stages of development. One-half to two-thirds
of the activity in brain and liver homogenates is located in the
soluble fraction. Supernatant activity increases in these organs to a
maximum during...
Levels of activity of glucose-6-phosphate- , 6-phosphogluconate- , and isocitric- dehydrogenase were measured in the
soluble and particulate fractions of heart, brain and liver at
several ages throughout the development of the chick embryo.
Compared to isocitric dehydrogenase, levels of glucosesphosphate-
, and 6-phosphogluconate- dehydrogenase are generally
low. This suggests...
Myelin formation in the chick embryo sciatic nerve was examined
during development by analysis of three myelin lipids (cholesterol,
phospholipids, and cerebrosides) and of a myelin related
enzyme (2', 3'-cyclic AMP 3'-phosphohydrolase). The role of the
Schwann cell in controlling the synthesis of these myelin components
was studied by in...
Phospholipids and cerebrosides of the normal chick sciatic
nerve were extracted by Folch procedures and separated with silica
gel thin-layer chromatography using various chloroform-methanol
solvents. The subsequent chemical assays were expressed as a function
of the developmental stages of the chick from 15 days of incubation
to 7 days after...
The DNAs (deoxyribonucleic acids)
of the thoracic tissue
of
the tobacco hornworm,
Manducta sexta, were studied
by physical
and chemical means. The adult
moth was considered to
be fully
formed two days after emergence from
the pupal case, as indicated
by its ability to fly and the integrity
of the...
Adenosine 3', 5' -cyclic monophosphate (cyclic AMP, cAMP) and guanosine 3', 5' -cyclic monophosphate (cyclic GMP, cGMP) were quantitated in the central nervous system (CNS) of the insect Manduca sexta by competitive protein binding techniques. The M. sexta CNS was found to contain a strikingly high level of cGMP, about...
Prothoracicotropic hormone has been partially purified
from whole heads of tobacco hornworm larvae, Manduca sexta.
Activity was followed with a larval bioassay. Activity was
purified 4400 fold from crude extracts. The hormone was
found to be a heat-stable polypeptide with an approximate
molecular weight of 25,000 Daltons. The hormone has...