Preparation of a cytochrome c-cytochrome oxidase complex
was achieved, using crystalline beef cytochrome c and purified
cytochrome oxidase from beef heart muscle, or from the
Keilin-Hartree preparation. Succinate oxidase was reconstituted
with the complex.
A mixture of cytochrome oxidase, solubilized with nonionic
detergent, and excess cytochrome c was exposed to...
The spectrophotometric method for the determination of the
acid nonextractable flavin in the proteolytic digest of the trichloroacetic
acid precipitate from the heart muscle preparation has been
examined for the interference of the degraded hemeproteins. The
proteolytic digest was chromatographically separated into the heme
and flavin fractions. Spectrophotometric determinations made...
Some kinetics of the oxygen requirement of amine oxidases
have been investigated using bovine plasma monoamine oxidase (PAO)
and pea seedling diamine oxidase (DAO). A system is described for
studying enzyme reactions under controlled oxygen tension and in an
O₂-free N₂ atmosphere.
O₂ Km values were determined at two different...
In order to determine the total number of cytochrome P-450 isozymes
present in a given species following various treatments, mature
male rats (Sprague-Dawley) were injected intraperitoneally with the
classic mixed-function oxidase inducers phenobarbital (PB), pregnenolone-
16a-carbonitrile (PCN), 3-methylcholanthrene (3-MC), or the appropriate
control vehicle.
Hepatic microsomes were solubilized and P-450...
Polyphenol oxidase (PPO) from d'Anjou pears was purified and some
molecular properties were studied. The extraction of PPO was
accomplished in the presence of the phenolic binder AG 2X-8 and Triton
X-100. Chlorophyll pigment was removed by chromatography resulting in
a clear, colorless enzyme extract. Three fractions of pear PPO...
Several aspects of the potent inhibition of bovine plasma amine
oxidase (PAO) by hydrazines were investigated by kinetic and preparative
means. The inhibition was classified as pseudo-irreversible
by the method of Ackerman and Potter (Proc. Soc. Exptl. Biol. Med.
72, 1 (1949), and was found to exhibit Zone B kinetic...