The purpose of the dissertation was to investigate
(certain aspects of) the host-parasite relationship between
the laboratory rabbit and the pathogen, Pasteurella multocida.
The focus of the present endeavor was to provide new
insight into this field of study by the adaptation of novel
microbiologic approaches and the application of...
Protective antigens were separated from a 2.5% saline extract of
Pasteurella multocida type 4 by chromatographic methods. The organism
was grown for 18 to 20 hours at 37°C on an agar medium containing 5%
heat-inactivated turkey serum. The bacterial growth was harvested in a
2.5% NaC1 solution and agitated for...
Enzyme-linked immunosorbent assays (ELISA) were developed to
detect rabbit antibodies against Pasteurella multocida. Antigens
for ELISA were prepared from 2.5% saline extracts of P. multocida
by chromatographic methods. From a crude saline extract of a type
3, 4 isolate designated as R11146, two protein peaks were obtained
by gel filtration...
Pathogenesis and immunity involved in fowl cholera were studied
with the turkey, one of the most susceptible natural hosts. The
causative agent, Pasteurella multocida, was analyzed in terms of its
virulence and immunogenic factors, using a highly virulent, encapsulated
strain P-1059.
Protective immunity was induced by lipopolysaccharide (LPS)-
protein complex...
Initial sites of localization and multiplication of Pasteurellamultocida were examined in chickens intravenously inoculated with varying doses of bacteria. Sections of lung, liver, kidney, bone marrow and spleen were prepared for light microscopic examination.Tissue sections were stained with the hematoxylin and eosin stain,Gram's stain or the avidin-biotin-peroxidase complex immunoenzymatictechnique. Of...
Monoclonal antibodies were developed against serotype 3, P-1059 strain of Pasteurella multocida. Enzyme-linked immunosorbent assays (ELISA) were used to detect antibodies in supernatants of hybridoma cells to either a surface protective (2.5 S) antigen prepared from a 2.5% saline extract or lipopolysaccharide (LPS) prepared by the phenol-water method. Forty two...
Pasteurella multocida causes diseases in many animal
species including fowl cholera, a septicemic disease of
poultry and other birds. Pathogenesis of the disease has
been studied by many investigators by the systemic
administration of the organism in poultry. However, only a
few studies have been done as to the respiratory...