Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, sulfur metabolism, biofilm formation, motility, and...
Zinc is an important nutrient but can be lacking in some soil environments, influencing the physiology of soil-dwelling bacteria. Hence, we studied the global effect of zinc limitation on the transcriptome of the rhizosphere biocontrol strain Pseudomonas protegens Pf-5 (formerly Pseudomonas fluorescens). We observed that the expression of the putative...
One of the most important micronutrients for bacterial growth is iron, whose bioavailability in soil is limited. Consequently, rhizospheric bacteria such as Pseudomonas fluorescens employ a range of mechanisms to acquire or compete for iron. We investigated the transcriptomic and proteomic effects of iron limitation on P. fluorescens Pf-5 by...
This paper examines ways in which natural resource management – NRM – may be decolonized in order to better serve Hawaiʻi communities. Expressed needs for diversity in NRM has brought about inclusion of traditional ecological knowledge in recent years. Here, traditional ecological knowledge is redefined as ʻIK, ʻike kupuna –...
The herbicide, Dacthal (dimethyl 2,3,5,6,-tetrachloroterephthalate) is
hydrolyzed to give the corresponding diacid and this derivative is a common
contaminant in ground water. In the Ontario region of eastern Oregon the
use of this herbicide on onions has resulted in the contamination of an
aquifer with this derivative. Since movement to...
Understanding seasonal changes in growth, survival, and movement rates is
crucial to salmonid management. These life history characteristics provide a context for
evaluation of management actions. We evaluated the life history of individually
marked Oncorhynchus mykiss gairdneri in the South Fork John Day River basin in
Northeastern Oregon. This thesis...
Conjugative plasmids are known to facilitate the acquisition and dispersal of genes contributing to the fitness of Pseudomonas spp. Here, we report the characterization of pA506, the 57-kb conjugative plasmid of Pseudomonas fluorescens A506, a plant epiphyte used in the United States for the biological control of fire blight disease...
Gene expression profiles of the biological control strain Pseudomonas protegens Pf-5 inhabiting pea seed surfaces were revealed using a whole-genome oligonucleotide microarray. We identified genes expressed by Pf-5 under the control of two global regulators (GacA and RpoS) known to influence biological control and secondary metabolism. Transcript levels of 897...
In spite of considerable efforts to restore natural runs of anadromous salmonids in the Pacific Northwest, they remain at risk of extirpation. Along with many other factors influencing the decline, stocking from hatcheries over the past hundred years is often suggested to be a major cause. The listing of over...