Vibrio marinus MP-1, an obligate psychrophilic marine
bacterium, was severely damaged when heat-shocked in the presence
of nutrients. Thermally induced leakage materials from cells tested
for in the medium were 260 mμ absorbing material (nucleic acids),
orcinol reacting material (RNA), ninhydrin reacting material (amino
acids), protein, malic dehydrogenase, and glucose-6-phosphate...
The marine psychrophilic bacterium Vibrio marinus MP-4
possessed a maximum temperature and hydrostatic pressure of
20 C and 425 atm for growth. The effects of temperatures of 21 and
25 C and hydrostatic pressures of 200, 400, 500, and 1,000 atm on
protein, RNA and DNA synthesis by V. marinus...
In this study the relationship between salinity and temperature
changes on microbial growth, enzyme induction and substrate uptake
were investigated. The obligately psychrophilic bacterium, V.
marinus MP-1 was grown for 48 hours at 15 C in a glucose-ammonium
medium (GAM) containing 0.4 M NaCl. Log-phase cells were harvested
and shifted...
The heat inactivation of the psychrophilic marine bacterium,
Ant-300, was investigated in terms of permeability control, glucose
uptake, and respiration. Extensive leakage of 14C-cellular material
occurred at 13 C, the maximum growth temperature of the organism.
Protein and RNA were released from the cells in significant
quantities. Inhibition of glucose...
Cells of the marine bacterium, Ant-300, accumulate loosely bound
amino acids during amino acid uptake. The fraction of amino acid taken
up that exists in the loosely bound state depends on the substrate in
question. Shock treatment studies indicate that, in general, the initial binding, uptake, and retention of amino...
Starved cells of Ant-300, a psychrophilic marine Vibrio, maintained
binding and transport systems for glutamic acid, arginine, proline, and
leucine. The uptake of these amino acids was inhibited by the uncoupler
2,4-dinitrophenol indicating that an energized membrane was necessary
for binding and was maintined in starved cells. The levels of...
Some enzymes of the citric acid cycle and glycolytic pathway
in cell-free extracts of Vibrio marinus MP-1 were compared for
thermal lability. After one hour of moderate temperature exposure,
enzymes of both pathways rapidly lost catalytic activity. For all
but one enzyme, 50 percent remaining activity occurred near an
averaged...
The effects of various physical parameters on the binding, uptake,
and catabolism of glucose in the marine psychrophilic bacterium,
Vibrio marinus, were studied. It was shown that shortly after
the cell was exposed to labeled glucose, the radioactive label became
rapidly associated with the cell fraction. It was also shown...
Cell populations of the marine bacterium ANT-300, from
either batch culture or continuous culture with dilution
rates ranging from D = 0.170 h⁻¹ (fast) to D = 0.015 h⁻¹ (slow) were monitored for 98 days under starvation.
Viability (CFU), acridine orange direct counts (AODC), and
optical density were measured. DNA,...