There is good evidence that the infection with avian myeloblastosis
virus BAI strain A causes myeloblasts to retain their primitive
nature. These cells are able to multiply and produce virus at a relatively
constant rate. From these observations, a major problem develops
in the study of the virus-induced leukemia, that...
Due to public and regulatory restrictions, genetic containment technology is critical for research and development of transgenic trees in forestry. A multi-year field trial demonstrated that the RNAi-LFY construct successfully induces sexual sterility in 6K10 poplar (Populus alba) without visible effects on vegetative development. However, its limited number of replicates...
This is the publisher’s final pdf. The published article is copyrighted by the author(s) and published by the Genetics Society of America. The published article can be found at: https://doi.org/10.1534/g3.116.027045
RNA isolated from BAI strain A (Myeloblastosis) Avian Tumor
Virus can be fractionated into two fractions on a sucrose density
gradient, one of higher molecular weight and one of low molecular
weight. The low molecular weight (LMW) virus RNA was found to
chromatograph the same as E. coli tRNA on...
In order to assess the importance of RNA synthesis in the induction
and antibody-producing phases of immune responses, actinomycin
D was used to inhibit the primary and secondary in vitro immune
responses of mouse spleen cells against heterologous erythrocytes.
The presence of actinomycin D (0.1 or 0.125 μg /ml) throughout...
The transcription process of infectious hematopoietic necrosis virus (IHNV), a rhabdovirus of salmonid fish, was studied both in vivo and in vitro. Polyadenylated RNA from IHNV infected-salmon cells was resolved electrophoretically into five bands, one of which was found to contain two mRNA species. The molecular weights and intracellular molar...
Messenger RNA (mRNA) therapy has massive potential to treat various genetic disorders by providing effective proteins to the diseased cells and tissues. Its therapeutic applications include protein replacement, genome editing, and immunotherapy. For successful mRNA treatment, mRNA must be delivered to the cytosol of target cells. One of the leading...