Reductive sulfate assimilation, the biological process by which sulfur-containing amino acids and key derivatives are synthesized from sulfate, is broadly shared among bacteria, fungi, and plants. It is the major, if not sole source of methionine and cysteine for Saccharomyces cerevisiae during wine fermentation. Two obligate intermediates formed in the...
Nutritional shift-up experiments have revealed many important
macromolecular interactions in bacteria. It has been shown that RNA
synthesis can be dissociated from protein and DNA synthesis. The
rates of protein synthesis were found to be, at a given temperature,
strictly dependent on the numbers of mature ribosomes present.
DNA synthesis...
The catabolism of glucose by proliferating and resting cells of
two strains of Saccharomyces cerevisiae has been examined, with
respect to the nature and participation of concurrent glucose pathways
by means of the radiorespirometric method. The nicotinamide
nucleotide content, and nicotinamide nucleotide oxidase activities
in these cells were also examined....
The inhibitory effect of 2-deoxy-D-glucose (2-DG) upon the
metabolism of glucose and fructose by proliferating cells of
Saccharomyces cerevisiae (OSU strain 2) has been examined. By
the use of radiotracer methods, particularly radiorespirometric
techniques, it has been possible to demonstrate that the rate of
hexose assimilation by yeast cells is...
Existing methods for the estimation of relative participation of
glucose catabolic pathways make use of yield data of respiratory
¹⁴CO₂ or intermediary ¹⁴C products derived from glucose substrates
specifically labeled with ¹⁴C. Cumulative yields of respiratory
¹⁴CO₂ observed at the end of time course of complete substrate
utilization are analyzed...
An assay is described to measure sterol uptake in
Saccharomyces cerevisiae. This assay involves specific
binding of radioactively labelled sterol to actively
growing yeast cells.
It was found that uptake does not occur in stationary
cultures and that some growth is necessary for FY3 , a
sterol auxotroph, to take...
The positions of the DNase I hypersensitive sites within the
galactose gene cluster of the yeast Saccharomyces cerevisiaa have
been determined. When yeast nuclei were incubated with DNase I, the
purified DNA restriction endonuclease digested and analyzed by
Southern hybridization, five hypersensitive regions were observed in
this cluster of three...