Selenomethionine and methionine have been postulated to be similarly
involved in protein synthesis. This thesis has evaluated this
hypothesis from a molecular and genetic approach. Selenomethionine
and methionine were incorporated into rabbit reticulocyte hemoglobin
at the same rates and in equal quantities. Each methionine demonstrated
competitive inhibition for its analog...
A method for the determination of selenium in biological
materials by gas-liquid chromatography has been developed and
successfully applied to kidney, heart, liver, and muscle tissue. The
initial digestion of animal tissue was accomplished in a mixture of
nitric and perchloric acids (3:1) until "water whiteness'' of solution
was achieved....
This study was conducted to investigate the distribution
of glutathione peroxidase (GSH-Px) and selenium in
semen of the boar, bull, ram and stallion, and in the reproductive
tissues of the boar, bull and ram. The effect
of repeated noncryoprotective freeze-thawing on GSH-Px
activity was also studied. Both hydrogen peroxide (H₂0₂)...
Studies were conducted to investigate the metabolism of
selenium (Se) , and the influences of Se on the metabolism of cadmium (Cd) and inorganic mercury (Hg) in rats, and in ram semen in
vitro. Se-deficient (-Se) or Se-adequate (+Se) rats were injected intraperitoneally with either ¹⁰⁹CdCl₂ (5 μCi/rat) or ²⁰³HgN0₃...
A great concern about the effect of oxygen in Se samples has been solved by a purification method called the distillation-with-Al method. The electrical conductivity of liquid Se prepared by this purification method shows Arrehnius behavior with a single activation energy and positive thermopower within the experimental temperature range. This...
In three separate trials rats were fed either a basal diet deficient
in selenium and vitamin E or the same diet supplemented with
one or both of these factors. In addition to these treatments in two
of the trials, ethoxyquin was included to provide adequate lipid antioxidant
protection and to...
A series of four trials were conducted to determine the influence
of selenium supplementation of a selenium- and vitamin E-deficient
basal diet on the absorption, excretion, tissue distribution
and placental transfer of a single dose of tritium-labeled α-tocopherol
in the rat.
In the first trial, the effect of supplementation of...
The selenium-responsive syndromes and the discovery of
Factor 3-selenium focused attention on selenium in metabolism. Two
hypotheses have been advanced to explain the metabolic effect of
selenium: 1) substitution or sparing of alpha tocopherol, and 2) a
primary role in metabolism. The first hypothesis is supported by
Scott and Desai,...