Experiments were conducted to determine the effect of rumen
fluid on live and motile spermatozoa of semen extended in yolk-citrate
and stored at 4°C. Preliminary work showed that levels of
rumen fluid below 10 percent were beneficial in keeping spermatozoan
activity up to 14 days in storage. For more revealing...
Investigations with ram semen were conducted to develop procedures
of cyrogenic preservation that would provide long-term
retention of fertilizing capacity in ram spermatozoa.
An isotonic extender consisting of egg yolk, sodium citrate,
lactose, glycerol, and a broad spectrum antibiotic was selected from
among several extenders tested in pilot studies. Using...
This study was conducted to investigate the distribution
of glutathione peroxidase (GSH-Px) and selenium in
semen of the boar, bull, ram and stallion, and in the reproductive
tissues of the boar, bull and ram. The effect
of repeated noncryoprotective freeze-thawing on GSH-Px
activity was also studied. Both hydrogen peroxide (H₂0₂)...
Studies were conducted to investigate the metabolism of
selenium (Se) , and the influences of Se on the metabolism of cadmium (Cd) and inorganic mercury (Hg) in rats, and in ram semen in
vitro. Se-deficient (-Se) or Se-adequate (+Se) rats were injected intraperitoneally with either ¹⁰⁹CdCl₂ (5 μCi/rat) or ²⁰³HgN0₃...
Successful fertilization of fresh steelhead trout, pink salmon, kokanee, chum salmon, fall chinook salmon, and coho salmon eggs with cryopreserved sperm is reported in this thesis. A mean percent fertilization greater than 50 percent was achieved for all species tested except chum salmon. Differences in sperm viability from donor males...