Dibenzo[def,p]chrysene (DBC) is a highly potent, but less prevalent, environmental carcinogen belonging to a class of compounds known as Polycyclic Aromatic Hydrocarbons (PAHs). They are highly ubiquitous and arise as a byproduct of natural and anthropogenic combustion processes. Previous studies have documented carcinogenic effects upon in utero exposure of PAHs....
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
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)
AhR (56.0˚C)
Acknowledgements
• LisbethSiddens
• Professor William Baird
Baird Lab
• Dr
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Full Text:
with environmental PAH mixtures
Andrew Larkina,b, LisbethK. Siddensb, Sharon K. Kruegerc, David E
Epidermal RNA samples from FVB/N mice treated with PAH standards and
environmental PAH mixtures were harvested and purified using Trizol extraction and
RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5),
indicating significant RNA degradation and the need to modify handling, collection, and
processing of skin...
Estrogen is a sex hormone responsible for the development and regulation of the reproductive system and secondary sex characteristics. They also play a critical role in the development of breast and uterine cancer. The formation of agonistic metabolites of estrogen, especially 16α-hydroxyestrone, have been found to have cancer-inducing effects meanwhile...
Cytochrome P450 1B1 (Cyp1B1) is a xenobiotic metabolizing enzyme, responsible for polycyclic aromatic hydrocarbon (PAH) oxidative metabolism. CYP1B1 metabolically activates several species of environmentally relevant procarcinogenic PAHs to reactive carcinogens. PAHs are aryl hydrocarbon receptor (AhR) agonists, leading to CYP1B1 upregulation. Though CYP1B1 is highly conserved across species, wild type...
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
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Hydrogen Peroxide
Siddens, L. K., Krueger, S. K., Henderson, M. C., & Williams, D. E. (2014).
Mammalian
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
Flavin-containing monooxygenase (FMO) oxygenates drugs/xenobiotics containing a
soft nucleophile through a C4a hydroperoxy-FAD intermediate. Human FMOs 1, 2 and
3, expressed in Sf9 insect microsomes, released 30-50% of O₂ consumed as H₂O₂
upon addition of NADPH. Addition of substrate had little effect on H₂O₂ production.
Two common FMO2 (the major...
Dibenzo[def,p]chrysene (DBC) is a polycyclic aromatic hydrocarbon (PAH) and an IARC classified 2A probable human carcinogen. Mouse progeny transplacentally exposed to DBC have been shown to develop lung and liver tumors, with liver tumors developing predominantly in males later in life. Sulforaphane (SFN) is an isothiocyanate that plays a crucial...
