Lactic Acid Bacteria (LAB), an important group of organisms in modern food production, are known to secrete a unique compound called exopolysaccharide (EPS). EPS is economically important because it enhances functional properties in food and may confer beneficial health effects to consumers. Novel strains of Lactococcus lactis, Streptococcus thermophilus, and...
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Janine E. Trempy
Lactic Acid Bacteria (LAB), an important group of organisms in
Bacterial contamination of food poses a great risk to human health worldwide. A
chromatophore cell-based biosensor, utilizing B. splendens erythrophore cells, is an
emerging technology that has shown potential to detect bacterial toxicity based on
function-dependent mechanisms. Previous studies have investigated the response of
erythrophore cells to foodborne pathogens, pesticides,...
Detection of both biological and chemical environmental toxicants is essential in the assessment of risk to human health. Cell-based biosensors are capable of activity- based detection of toxicity. Chromatophore cells, responsible for the pigmentation of poikilothermic animal, have shown immense potential as cell-based biosensors in the detection of a broad...
This research effort examined Traditional Ecological Knowledge (TEK) of Siletz tribal members of the environment they live and/or lived in, and how both Western societal beliefs and land reduction to the initially established reservation location affected and altered how the area TEK has been maintained and passed down. The theory...
Lactococcus lactis subsp. cremoris Ropy 352 and L. lactis
subsp. cremoris Hollandicus produce an exopolysaccharide (EPS) that
imparts commercially desirable textural and rheological properties
to fermented milk products. This ropy phenotype is expressed under
specific environmental conditions. A mucoid EPS phenotype, also
expressed under specific environmental conditions, but not involved...
Rapid degradation of certain short lived "timing" proteins is
an effective mechanism for cells to control important regulatory
pathways. The mechanisms by which regulatory proteases
recognize their substrates are not well understood. Escherichia coli
Lon, an energy dependent protease highly conserved in many
prokaryotes and eukaryotes provides a model system...
The genes coding for the expression of a ropy exopolysaccharide responsible for commercially desirable textural and rhealogical traits in fermented milk products by a natural lactococcal ropy isolate were sought. Using a transposon mutagenesis vector, pGh9:ISS1, three mutants lacking expression of the ropy exopolysaccharide were isolated. One of the mutants...
The mechanisms of substrate recognition by regulatory proteases are not well understood. Presently, two opposing models have arisen to describe E. coil Lon's ability to discriminate between substrates: one suggests the N-terminus involvement while the second suggests the C-terminus involvement. In this project, the role of the C-terminal domain as...
Afghanistan is one of the poorest countries in the world. Most of the people live in villages below the poverty line, and do not have access to basic health care and a primary education. Living standards are very low, and surviving day to day is a lifetime struggle. Most villages...