Graduate Thesis Or Dissertation
 

Prevalence and distribution of calicivirus neutralizing antibody in selected U.S. cattle

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/cv43p051v

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  • Serum samples from 1,046 Kansas cattle were examined for the presence of neutralizing antibody to caliciviruses known to be circulating in the marine environment. All sera were from farm-origin cattle, with the number tested being proportionate to the total number of cattle in each county. These sera were selected in a random manner from blood samples routinely submitted to the Kansas State/Federal Brucellosis Laboratory in 1988. The majority of animals represented by the sample were adult females of mixed breed. The sera were tested for the presence of antibody to the San Miguel sea lion virus (SMSV) types; 1, 5, 7,.8, 14, and the Tillamook calicivirus serotype (BCV Bos-1). Antibody was detected by the microneutralization procedure with Vero cells as the indicator system. Using a 1:10 serum dilution, neutralizing antibodies were found in the sample to all serotypes except SMSV-1 with prevalence rates ranging from .3% to 32%. All neutralization screen tests were read to,a 100% end point (EP) using a 100-421 tissue culture infective dose 50% end point (TCID50) of challenge virus. Due to the low antibody levels, prevalence values were found to be very dependent upon the virus challenge dose and the antibody EP used in the test. Antibody titers to four serotypes ranged from 1:10 to 1:1280 with most falling in the 1:10 to 1:20 range using a 50% antibody EP and a 100-421 TCID5O of challenge virus. Several sera were found to possess neutralizing antibody to multiple serotypes. Antibody to SMSV-5 was shown to be well distributed throughout Kansas indicating that widespread exposure to this marine calicivirus had occurred.
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