Undergraduate Thesis Or Project
 

HHMI Report 2011 Andres Alvarez.docx

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  • Many plant-associated Gram-negative bacteria, such a Pseudomonas syringae pv tomato DC3000 (PtoDC3000), use a type III secretion system (T3SS) to establish a host-microbe relationship with compatible plants. The T3SS resembles a molecular injection mechanism that allows the bacteria to deliver type III effectors (T3Es) directly into the cell of the host plant. These effectors interact with native proteins inside the plant cell in order to dampen the plant’s immunity mechanisms. We are determining the interaction of two T3Es from PtoDC3000 and their candidate target proteins using a yeast two-hybrid assay.
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