http://hdl.handle.net/1957/18339 2013-05-26T01:33:37Z 2013-05-26T01:33:37Z Ostertag-Hill, Claire http://hdl.handle.net/1957/38726 2013-05-24T23:52:15Z 2013-05-24T00:00:00Z

Investigation of the genetic differences between bovine herpesvirus type 1 variants and vaccine strains Ostertag-Hill, Claire Although an efficacious bovine herpesvirus type-1 (BHV-1) vaccine has been used for many years, BHV-1 vaccine-related abortion and respiratory disease have been occurring in a rising number of herds. To investigate whether isolates from BHV-1 vaccination with adverse effects are identical to the wild type virus or vaccine virus, regions of the thymidine kinase (TK) gene prone to mutation were examined. Two sets of PCR primers were used to investigate two unique polymorphisms of the TK gene in 35 BHV-1 isolates. The DNA polymorphisms in the TK gene were determined by Sanger sequencing of PCR amplicons and the sequence was analyzed with Geneious software. All BHV-1 isolates and vaccine were found to have TK genes that are different from the wild type virus. Over 50% of the sequenced BHV-1 isolate has TK genes that are different from vaccine strains, with 27% having genetic variation in region 1 of TK and 55% in region 2 of TK. This study suggests that BHV-1 isolates have genetic similarity with vaccine virus but have variation within TK genes, which may have resulted from mutations occurred within the vaccinated animals or from infection with a strain similar to the vaccine virus.

2013-05-24T00:00:00Z Huang, Kenneth http://hdl.handle.net/1957/38700 2013-05-21T20:01:54Z 2013-05-21T00:00:00Z

Effect of Temperature on Osmotic Tolerance Limits for Adherent Endothelial Cells Huang, Kenneth Cryopreservation depends on the equilibration of cells with high concentration of cryoprotective agents (CPAs). However, the addition of CPAs can induce damages to the cells. One of the damages is due to too much shrinkage or swell of the cell volume which lead to the cell death during the addition or removal of CPAs procedure. Therefore, it is important to optimize the tolerance limits of cell volume. The goal of this research is to investigate the effect of temperature on the tolerance limits of cell volume. The experiments are carried out with Bovine Pulmonary Artery Endothelial Cells (BPAEC), which are exposed to different NaCl treatment for fifteen minutes. The metabolic rates are later measured by the presto blue viability reagent. There are finding that at low temperature data there is a relatively high cell survival when exposing to extreme hypotonic or hypertonic solutions; and relatively low cell survival for the high temperature data set.

2013-05-21T00:00:00Z Martinez, Angel M. http://hdl.handle.net/1957/38189 2013-04-16T00:34:15Z 2013-04-15T00:00:00Z

The Relationship Between Institutional Internalization and Organic Solidarity Martinez, Angel M. This paper will focus on the relationship between institutional internalization and organic solidarity. Being that there is a wide verity of different institutions, this writing will focus primarily on only religion, politics, and education. The internalization of the individual from each of these institutions greatly effects their beliefs, desires, and action, which as a result branches out into society from a mechanical solidarity operation into a more organic solidarity operation. Organic solidarity will depend on individuals in their respected association, to internalize their specific role. A control variable was used in this research to detect any spurious relationships that may change the final data and to avoid a type I or II error. The controlled variables used were five different categorizes of religious affiliation. Without the controlled variable the significance of the relationship between Institutional Internalization and Organic Solidarity was only .179. With the five controlled variables applied there was no significance found. With an additional Regression Analysis calculated there was minimal significance found with specific variables.

2013-04-15T00:00:00Z Goodrich, Danielle http://hdl.handle.net/1957/38016 2013-05-21T21:13:54Z 2013-04-04T00:00:00Z

Discovering the roles of myosin and exocyst proteins in cell expansion of Arabidopsis thaliana Goodrich, Danielle The overall objective of this research was to determine which, if any, myosin XI proteins and exocyst proteins play a role in growth of Arabidopsis thaliana hypocotyls and root hairs. This involved the study of whether exocyst and myosin proteins work together during cell expansion within these two plant parts. Another objective was to determine why some protein mutants grew shorter hypocotyls or root hairs than the Col-0 Wildtype (WT). To explore the answers to these questions, I performed many hypocotyl and root hair measurements of WT and various protein mutants. The results indicated that shortened hypocotyls are due to shortened cells in the hypocotyls rather than less cells. Results also pointed toward the theory that shortened root hair cells are due to slower growth rather than growth to a certain point before it is stopped.

2013-04-04T00:00:00Z