http://hdl.handle.net/1957/1932 2013-05-21T23:00:18Z 2013-05-21T23:00:18Z Grevstad, Fritzi Shaw, Richard Bourchier, Robert Sanguankeo, Paolo Cortat, Ghislaine Reardon, Richard C. http://hdl.handle.net/1957/38691 2013-05-21T16:16:43Z 2013-01-12T00:00:00Z

Efficacy and host specificity compared between two populations of the psyllid Aphalara itadori, candidates for biological control of invasive knotweeds in North America Grevstad, Fritzi; Shaw, Richard; Bourchier, Robert; Sanguankeo, Paolo; Cortat, Ghislaine; Reardon, Richard C. Invasive knotweeds are large perennial herbs in the Polygonaceae in the genus Fallopia that are native to Asia and invasive in North America. They include Fallopia japonica (Japanese knotweed), F. sachalinensis (giant knotweed), and a hybrid species F. x bohemica (Bohemian knotweed). Widespread throughout the continent and difficult to control by mechanical or chemical methods, these plants are good targets for classical biological control. We examined the suitability of two populations of the psyllid Aphalara itadori from Japan as biological control agents by comparing their impact on the target weeds and assessing their fundamental host ranges. Both populations were capable of halting knotweed plant growth and reducing both above and below ground biomass by more than 50% in just 50 days. Moreover, the psyllids caused mortality of several of the plants during this period. The two populations differed markedly in their reproductive potential on the different knotweed species. The Kyushu psyllid performed best on F. japonica and F. bohemica and the Hokkaido psyllid performed best on F. sachalinensis. Both were found to be specialized to knotweeds, with only very low occurrence of development on a small number of related non-target plant species. For the few non-target plant species that supported development, choice tests and multi-generational tests were used to further evaluate the likelihood of non-target host use. We conclude that A. itadori would be both effective and low risk as a biological control agent for invasive knotweeds and that both the Kyushu and Hokkaido populations may be needed to effectively control the entire knotweed species complex. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by Elsevier and can be found at: http://www.elsevier.com/.

2013-01-12T00:00:00Z Lim, Chee Kent Penesyan, Anahit Hassan, Karl A. Loper, Joyce E. Paulsen, Ian T. http://hdl.handle.net/1957/38689 2013-05-21T15:52:09Z 2013-02-22T00:00:00Z

Effect of Tannic Acid on the Transcriptome of the Soil Bacterium Pseudomonas protegens Pf-5 Lim, Chee Kent; Penesyan, Anahit; Hassan, Karl A.; Loper, Joyce E.; Paulsen, Ian T. Tannins are a diverse group of plant-produced, polyphenolic compounds with metal-chelating and antimicrobial properties that are prevalent in many soils. Using transcriptomics, we determined that tannic acid, a form of hydrolysable tannin, broadly affects the expression of genes involved in iron and zinc homeostases, sulfur metabolism, biofilm formation, motility, and secondary metabolite biosynthesis in the soil-and rhizosphere-inhabiting bacterium Pseudomonas protegens Pf-5. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by American Society for Microbiology and can be found at: http://aem.asm.org/.

2013-02-22T00:00:00Z Van Auken, Kimberly Fey, Petra Berardini, Tanya Z. Dodson, Robert Cooper, Laurel Li, Donghui Chan, Juancarlos Li, Yuling Basu, Siddhartha Muller, Hans-Michael Chisholm, Rex Huala, Eva Sternberg, Paul W. WormBase Consortium http://hdl.handle.net/1957/38585 2013-05-15T16:12:59Z 2012-11-17T00:00:00Z

Text mining in the biocuration workflow: applications for literature curation at WormBase, dictyBase and TAIR Van Auken, Kimberly; Fey, Petra; Berardini, Tanya Z.; Dodson, Robert; Cooper, Laurel; Li, Donghui; Chan, Juancarlos; Li, Yuling; Basu, Siddhartha; Muller, Hans-Michael; Chisholm, Rex; Huala, Eva; Sternberg, Paul W.; WormBase Consortium WormBase, dictyBase and The Arabidopsis Information Resource (TAIR) are model organism databases containing information about Caenorhabditis elegans and other nematodes, the social amoeba Dictyostelium discoideum and related Dictyostelids and the flowering plant Arabidopsis thaliana, respectively. Each database curates multiple data types from the primary research literature. In this article, we describe the curation workflow at WormBase, with particular emphasis on our use of text-mining tools (BioCreative 2012, Workshop Track II). We then describe the application of a specific component of that workflow, Textpresso for Cellular Component Curation (CCC), to Gene Ontology (GO) curation at dictyBase and TAIR (BioCreative 2012, Workshop Track III). We find that, with organism-specific modifications, Textpresso can be used by dictyBase and TAIR to annotate gene productions to GO's Cellular Component (CC) ontology. This is the publisher’s final pdf. The published article is copyrighted by Oxford University Press and can be found at: http://www.oxfordjournals.org/

2012-11-17T00:00:00Z Hartney, Sierra L. Mazurier, Sylvie Girard, Maeva K. Mehnaz, Samina Davis, Edward W. II Gross, Harald Lemanceau, Philippe Loper, Joyce E. http://hdl.handle.net/1957/38451 2013-05-06T17:08:49Z 2013-02-01T00:00:00Z

Ferric-Pyoverdine Recognition by Fpv Outer Membrane Proteins of Pseudomonas protegens Pf-5 Hartney, Sierra L.; Mazurier, Sylvie; Girard, Maeva K.; Mehnaz, Samina; Davis, Edward W. II; Gross, Harald; Lemanceau, Philippe; Loper, Joyce E. The soil bacterium Pseudomonas protegens Pf-5 (previously called P. fluorescens Pf-5) produces two siderophores, enantio-pyochelin and a compound in the large and diverse pyoverdine family. Using high-resolution mass spectroscopy, we determined the structure of the pyoverdine produced by Pf-5. In addition to producing its own siderophores, Pf-5 also utilizes ferric complexes of some pyoverdines produced by other strains of Pseudomonas spp. as sources of iron. Previously, phylogenetic analysis of the 45 TonB-dependent outer membrane proteins in Pf-5 indicated that six are in a well-supported clade with ferric-pyoverdine receptors (Fpvs) from other Pseudomonas spp. We used a combination of phylogenetics, bioinformatics, mutagenesis, pyoverdine structural determinations, and cross-feeding bioassays to assign specific ferric-pyoverdine substrates to each of the six Fpvs of Pf-5. We identified at least one ferric-pyoverdine that was taken up by each of the six Fpvs of Pf-5. Functional redundancy of the Pf-5 Fpvs was also apparent, with some ferric-pyoverdines taken up by all mutants with a single Fpv deletion but not by a mutant having deletions in two of the Fpv-encoding genes. Finally, we demonstrated that phylogenetically related Fpvs take up ferric complexes of structurally related pyoverdines, thereby establishing structure-function relationships that can be employed in the future to predict the pyoverdine substrates of Fpvs in other Pseudomonas spp. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by American Society For Microbiology and can be found at:http://www.asm.org/.

2013-02-01T00:00:00Z