http://hdl.handle.net/1957/13589 Sat, 25 May 2013 10:38:39 GMT 2013-05-25T10:38:39Z http://hdl.handle.net/1957/36349 Genome Sequence of Nitrosomonas sp. Strain AL212, an Ammonia-Oxidizing Bacterium Sensitive to High Levels of Ammonia Yuichi, Suwa; Norton, Jeanette M.; Bollmann, Annette; Klotz, Martin G.; Stein, Lisa Y.; Laanbroek, Hendrikus J.; Arp, Daniel J.; Goodwin, Lynne A.; Chertkov, Olga; Held, Brittany; Bruce, David; Detter, J. Chris; Detter, Janine C.; Tapia, Roxanne; Han, Cliff S. Nitrosomonas sp. strain AL212 is an obligate chemolithotrophic ammonia-oxidizing bacterium (AOB) that was originally isolated in 1997 by Yuichi Suwa and colleagues. This organism belongs to Nitrosomonas cluster 6A, which is characterized by sensitivity to high ammonia concentrations, higher substrate affinity (lower K[subscript m]), and lower maximum growth rates than strains in Nitrosomonas cluster 7, which includes Nitrosomonas europaea and Nitrosomonas eutropha. Genome-informed studies of this ammonia-sensitive cohort of AOB are needed, as these bacteria are found in freshwater environments, drinking water supplies, wastewater treatment systems, and soils worldwide. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. This is the publisher’s final pdf. The published article is copyrighted by American Society for Microbiology. and can be found at: http://www.asm.org/. Thu, 01 Sep 2011 00:00:00 GMT http://hdl.handle.net/1957/36349 2011-09-01T00:00:00Z http://hdl.handle.net/1957/36126 Landscape-Scale Rehabilitation of Medusahead (Taeniatherum caput-medusae)- Dominated Sagebrush Steppe Sheley, Roger L.; Vasquez, Edward A.; Chamberlain, Anna-Marie; Smith, Brenda S. Producers facing infestations of invasive annual grasses regularly voice the need for practical revegetation strategies that can be applied across broad landscapes. Our objective was to determine the potential for scaling up the single-entry approach for revegetating medusahead-infested rangeland to broader, more heterogeneous landscape-scale revegetation of winter annual grass–infested rangeland. We hypothesized, when applied on a highly variable landscape scale, the combination of imazapic and seeding would provide highest abundance of perennial grasses and lowest amount of annual grasses. Treatments included a control, seeding of crested wheatgrass (‘Hycrest’) and Sandberg's bluegrass, spraying (60 g ai ha⁻¹ imazapic), and a simultaneously applied combination of spraying and seeding. The HyCrest and Sandberg's bluegrass seeding rates were 19 and 3.4 kg ha⁻¹, respectively. The treatments were applied to large plots (1.4 to 8 ha) and replicated five times, with each replication located in different watersheds throughout southeastern Oregon. This study shows that the single-entry approach can be scaled up to larger landscapes, but variation within establishment areas will likely be high. This procedure should reduce the costs over multientry treatment applications and make revegetating annual grass–infested rangeland across landscapes more affordable. This is the publisher’s final pdf. The published article is copyrighted by the Weed Science Society of America and can be found at: http://wssajournals.org/loi/ipsm. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. Mon, 01 Oct 2012 00:00:00 GMT http://hdl.handle.net/1957/36126 2012-10-01T00:00:00Z http://hdl.handle.net/1957/35102 Developmental Time, Longevity, and Lifetime Fertility of Three Introduced Parasitoids of the Mealybug Paracoccus marginatus (Hemiptera: Pseudococcidae) Amarasekare, Kaushalya G.; Mannion, Catharine M.; Epsky, Nancy D. Developmental time, longevity, and lifetime fertility of three previously introduced parasitoids (Acerophagus papayae Noyes and Schauff, Anagyrus loecki Noyes and Menezes, and Pseudleptomastix mexicana Noyes and Schauff) (Hymenoptera: Encyrtidae) of the mealybug Paracoccus marginatus Williams and Granara de Willink (Hemiptera: Pseudococcidae) were studied in the laboratory to understand the outcome of their recovery in field studies conducted in the United States. The developmental time of both male and female A. papayae and A. loecki was shorter than the developmental time of male and female P. mexicana. Male parasitoids of all three species had a shorter developmental time than their females. All parasitoids had a shorter developmental time in adult-female mealybugs than in second instars. Mating status (unmated and mated) had no effect on the male longevity. Unmated and mated females that were not allowed to oviposit had similar longevity and lived longer than those that were allowed to oviposit. Virgin females produced male only progeny with higher number of males from A. loecki or P. mexicana than from A. papayae. The number of females and the cumulative progeny was smaller for A. papayae than for A. loecki or P. mexicana. The progeny sex ratio (proportion of females) was not different among the parasitoids. A. papayae had the shortest reproductive period followed by A. loecki and P. mexicana, respectively. This information is important in evaluating the efficiency, recovery and establishment of A. papayae, A. loecki, and P. mexicana. This is the publisher’s final pdf. The published article is copyrighted by the Entomological Society of America and can be found at: http://www.entsoc.org/Pubs/Periodicals/EE. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work. • This article is the copyright property of the Entomological Society of America and may not be used for any commercial or other private purpose without specific written permission of the Entomological Society of America. Mon, 01 Oct 2012 00:00:00 GMT http://hdl.handle.net/1957/35102 2012-10-01T00:00:00Z http://hdl.handle.net/1957/29248 Population Dynamics of the Beet Leafhopper in Northeastern Oregon and Incidence of the Beet Leafhopper-Transmitted Virescence Agent Phytoplasma Crosslin, J. M.; Rondon, S. I.; Hamm, P. B. Beet leafhoppers were collected weekly on yellow sticky traps placed at 36 locations in Morrow and Umatilla Counties in northeastern Oregon in April through November 2007, 2008, and 2009. Insects were counted, collected, and a subset of the insects was tested for the presence of the beet leafhopper-transmitted virescence agent phytoplasma, the causal agent of potato purple top disease in this region. Beet leafhoppers were present throughout the sampling period and the number of insects collected peaked in June of each year with smaller peaks in July and October. Of the 804 insects tested for phytoplasma in 2007, 2008, and 2009, 21, 18, and 22% tested positive for phytoplasma, respectively. Most of the phytoplasma-positive insects were collected from mid-June through July. Positive insects, however, were collected as late as 13 November in 2007 and 2008. These data indicate that a relatively high proportion of the beet leafhoppers in this area are harboring the phytoplasma. Therefore, the potential for development of purple top disease of potatoes from migrating beet leafhoppers in this important potato producing region is quite high and measures to control this pest throughout the growing season are probably necessary in order to reduce disease pressure. Wed, 01 Feb 2012 00:00:00 GMT http://hdl.handle.net/1957/29248 2012-02-01T00:00:00Z