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Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1

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dc.creator Thomas, William J.
dc.creator Chang, Jeff
dc.creator Thireault, Caitlin A.
dc.creator Kimbrel, Jeffrey
dc.date.accessioned 2010-09-14T17:08:35Z
dc.date.available 2010-09-14T17:08:35Z
dc.date.issued 2009-08-06
dc.identifier.citation William J. Thomas1,2,†, Caitlin A. Thireault1,†, Jeffrey A. Kimbrel1,2 and Jeff H. Chang1,2,3,* (2009) Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1 The Plant Journal doi: 10.1111/j.1365-313X.2009.03998.x en
dc.identifier.uri http://hdl.handle.net/1957/18385
dc.description.abstract SUMMARY Many Gram-negative bacteria use a type III secretion system (T3SS) to establish associations with their hosts. The T3SS is a conduit for direct injection of type-III effector proteins into host cells, where they manipulate the host for the benefit of the infecting bacterium. For plant-associated pathogens, the variations in number and amino acid sequences of type-III effectors, as well as their functional redundancy, make studying type-III effectors challenging. To mitigate this challenge, we developed a stable delivery system for individual or defined sets of type-III effectors into plant cells. We used recombineering and Tn5-mediated transposition to clone and stably integrate, respectively, the complete hrp/hrc region from Pseudomonas syringae pv. syringae 61 into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1. We describe our development of Effector-to-Host Analyzer (EtHAn), and demonstrate its utility for studying effectors for their in planta functions. en
dc.publisher Journal compilation ª 2009 Blackwell Publishing Ltd, The Plant Journal, (2009), doi: 10.1111/j.1365-313X.2009.03998.x en
dc.subject Pseudomonas syringae en
dc.subject type-III secretion system en
dc.subject type-III effectors en
dc.subject hrp/hrc en
dc.subject plant defense en
dc.subject virulence en
dc.title Recombineering and stable integration of the Pseudomonas syringae pv. syringae 61 hrp/hrc cluster into the genome of the soil bacterium Pseudomonas fluorescens Pf0-1 en
dc.type Technical Report en


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