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Analysis of the thermophilic cyanobacterium Thermosynechococcus elongatus as a model organism for carbon sequestration, biofuel, and biomaterial production

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dc.contributor.advisor Ely, Roger
dc.creator Eberly, Jed O.
dc.date.accessioned 2010-11-05T22:17:25Z
dc.date.available 2010-11-05T22:17:25Z
dc.date.copyright 2010-10-21
dc.date.issued 2010-11-05
dc.identifier.uri http://hdl.handle.net/1957/19105
dc.description Graduation date: 2011 en_US
dc.description.abstract The thermophilic cyanobacterium Thermosynechococcus elongatus was examined for the ability to sequester CO₂ while producing hydrogen (H₂), polyhydroxybutyrate (PHB), lipids, and glycogen. H₂ was produced at a maximum rate of 188 nmol H₂ mg Chl a⁻¹ hr⁻¹. Hydrogen production occurred in the presence of methyl viologen but the cells were not able to catalyze deuterium (D₂) exchange. Screening assay studies showed that the inhibitors DCMU, DBMIB, PCP, and malonate did not effect H₂ production while H₂ production was completely inhibited when KCN was present. The physiological response and genome analysis of T. elongatus indicates that the enzyme responsible for H₂ production may differ from the Fe-only or [NiFe] bidirectional H₂ase enzymes commonly found in green algae and in other species of cyanobacteria. T. elongatus was also analyzed for the ability to grow on 5-150 mM dissolved inorganic carbon (DIC) and 0-30% CO₂. The effect of DIC and CO₂ concentrations on PHB, lipids, and glycogen was also measured. Glycogen and PHB were the primary carbon reserves depending on carbon availability. The highest glycogen concentration was 24.12% (w/w) with 150 mM DIC while the greatest PHB concentration was 14.5% (w/w) in cultures grown with 0% supplemental CO₂. Lipid content did not vary significantly with carbon concentrations and averaged around 15-20% (w/w) for all culture conditions. Cultures grown on CO₂ showed no difference in glycogen content over the range of CO₂ concentrations tested. Bioreactor experiments showed that T. elongatus was able to grow on up to 20% CO₂ while sequestering a maximum of 1.15 g L⁻¹ over the 9 day experiment. The maximum productivity and CO₂ fixation rates were 0.09±0.01 mg d⁻¹ and 0.17±0.01 mg ml⁻¹ d⁻¹, respectively, for cultures grown on 20% CO₂. en_US
dc.language.iso en_US en_US
dc.subject Thermophile en_US
dc.subject Thermosynechococcus en_US
dc.subject Biofuel en_US
dc.subject Biomaterials en_US
dc.title Analysis of the thermophilic cyanobacterium Thermosynechococcus elongatus as a model organism for carbon sequestration, biofuel, and biomaterial production en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Doctor of Philosophy (Ph. D.) in Biological and Ecological Engineering en_US
dc.degree.level Doctoral en_US
dc.degree.discipline Engineering en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Chaplen, Frank
dc.contributor.committeemember Liu, Hong
dc.contributor.committeemember Dolan, Mark
dc.contributor.committeemember Geller, Bruce
dc.contributor.committeemember Fern, Xiaoli


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