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Effect of the calpain inhibitor E-64-d on the degradation of α-fodrin in damaged muscle

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dc.contributor.advisor Widrick, Jeffrey J.
dc.creator Boyd, Jeffrey
dc.date.accessioned 2006-05-23T17:29:31Z
dc.date.available 2006-05-23T17:29:31Z
dc.date.copyright 2006-04-06
dc.date.issued 2006-05-23T17:29:31Z
dc.identifier.uri http://hdl.handle.net/1957/1940
dc.description Graduation date: 2006
dc.description.abstract We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain inhibitor. Both EDL and SOL muscles displayed significant differences (p<0.003 and p<0.002 respectively) between the raw and normalized 150 and 145 kDa α-fodrin fragments of the DMSO + E-64-d compared to the other bath treatments. Based on our model of exercise-induced muscle damage, we expected to see greater levels of 150 and 145 kDa α-fodrin fragments in those muscles that performed the eccentric protocol. However, there was no evidence that eccentric muscle damage increased the levels of 150 and 145 kDa α-fodrin fragments over the levels observed in the isometric trials. These findings suggest that the magnitude of damage was insufficient to activate calpains. en
dc.format.extent 464645 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en
dc.subject calpain en
dc.subject E-64-d en
dc.subject eccentric muscle damage en
dc.subject.lcsh Calpain -- Physiological effect en
dc.subject.lcsh Muscles -- Wounds and injuries en
dc.subject.lcsh Cytoskeletal proteins en
dc.subject.lcsh Calcium -- Antagonists en
dc.title Effect of the calpain inhibitor E-64-d on the degradation of α-fodrin in damaged muscle en
dc.type Thesis en
dc.degree.name Master of Science (M.S.) in Exercise and Sport Science en
dc.degree.level Master's en
dc.degree.discipline Health and Human Sciences en
dc.degree.grantor Oregon State University en
dc.contributor.committeemember Wilcox, Anthony
dc.contributor.committeemember Bella, Deborah
dc.contributor.committeemember Levenson, Rick


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