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Differential gene expression in the pregnant bovine corpus luteum during maternal recognition of pregnancy

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dc.contributor.advisor Menino, Alfred R. Jr.
dc.creator DuBay, Kristi M.
dc.date.accessioned 2010-12-16T23:38:34Z
dc.date.available 2010-12-16T23:38:34Z
dc.date.copyright 2010-12-03
dc.date.issued 2010-12-16
dc.identifier.uri http://hdl.handle.net/1957/19571
dc.description Graduation date: 2011 en_US
dc.description.abstract Interferon tau (IFN-τ) is the pregnancy recognition signal secreted by the trophectodermal cells of the developing bovine embryo that prevents luteolysis and maintains critical progesterone production. Recent evidence has suggested in addition to the mechanism of action identified in the uterus, there may be a direct effect of IFN-τ on the corpus luteum (CL). The objectives of this study were to generate a gene expression profile for the CL during maternal recognition of pregnancy, identify all genes where expression is modulated, and characterize the direction and magnitude of gene expression. To accomplish this two CL were collected from each of five cows on Day 14 of the estrous cycle and pregnancy (Day 0 = onset of estrus). Affymetrix Bovine GeneChip® microarrays were used to identify genes significantly up- or down-regulated in pregnant compared to non-pregnant cows. Microarray analysis detected 29 up-regulated and 6 down-regulated genes with a ≥1.5-fold change (P <0.05). From the differentially expressed genes, four were selected for validation with real-time RT-PCR. An additional 17 genes related to prostaglandin synthesis, growth hormone, IGF-1, interferon-tau-related genes, and hormone receptors were chosen for investigation with real-time RT-PCR. Analysis of the PCR results identified four genes, three involved in prostaglandin synthesis and the gene encoding the LH receptor, whose expression was (P <0.05) down-regulated in the pregnant CL during maternal recognition of pregnancy. These results suggest that the presence of the embryo on Day 14 of pregnancy cause the CL to become less competent in intraluteal prostaglandin synthesis, thereby contributing to the extension of luteal lifespan. en_US
dc.language.iso en_US en_US
dc.subject Corpus Luteum en_US
dc.subject Gene Expression en_US
dc.subject Bovine en_US
dc.subject.lcsh Cows -- Pregnancy en_US
dc.subject.lcsh Cows -- Embryos -- Physiology en_US
dc.subject.lcsh Cows -- Generative organs -- Physiology en_US
dc.subject.lcsh Cows -- Reproduction -- Endocrine aspects en_US
dc.subject.lcsh Corpus luteum en_US
dc.subject.lcsh Gene expression en_US
dc.title Differential gene expression in the pregnant bovine corpus luteum during maternal recognition of pregnancy en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Animal Sciences en_US
dc.degree.level Master's en_US
dc.degree.discipline Agricultural Sciences en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Bobe, Gerd
dc.contributor.committeemember Chappell, Patrick E.
dc.contributor.committeemember Tornquist, Susan J.


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