The human cathelicidin antimicrobial peptide (CAMP) is a broad spectrum microbicidal agent and modulator of both the innate and adaptive immune system. It is induced by 1,25-dihydroxyvitamin D (1,25(OH)₂D₃) through activation of the vitamin D receptor (VDR) and primary bile salts through activation of the xenobiotic nuclear receptor farnesoid X...
The effects of pH on the torsional flexibility of DNA bound to a
nucleosome core particle were investigated by studying the time-resolved
fluorescence anisotropy decays of ethidium bromide intercalated into the DNA
of the core particle. As the torsional flexibility of DNA is affected by the
presence of an intercalating...
By using immobilized trypsin, I have been able to
prepare well-defined, stable trypsinized nucleosomes. The
difficulties of lacking of control in the extent of
trypsinization, which were encountered in previous studies
with the use of free trypsin, have been eliminated. The
nucleosomes and oligonucleosomes prepared by immobilized
trypsin are suitable...
Ribonucleoside diphosphate reductase (RR) from vaccinia virus was recently cloned and overexpressed rn Escherichia coli. The amino acid sequence identities of the small and large subunits between the mouse and the vaccinia virus reductase are approximately 80 and 72 percent, respectively. In addition, vaccinia virus RR displays similar complex allosteric...
The ability to faithfully replicate DNA is dependent upon the maintenance
and regulation of its precursors, the deoxyribonucleoside triphosphates.
Enzymes encoded by the bacteriophage T4 have been widely used as models
of biochemical processes. A body of evidence supports the concept that the
bacteriophage T4 enzymes involved in deoxyribonucleotide biosynthesis...
For ras gene mutation analysis in the rainbow trout (Oncorhynchus
mykiss) model system, a partial trout ras sequence was identified
using the polymerase chain reaction (PCR). Two synthetic
oligonucleotides based on rat K-ras gene sequence were used as
primers for the PCR procedure. A 90 base pair (bp) sequence,
referred...
The work in this thesis has provided conclusive genetic evidence
that "panhandle" intermediates form during adenovirus replication.
Adenovirus chromosomes lacking 51 by from their left -hand termini
are infectious and capable of regenerating the missing origin
sequence. Yet if an entire inverted terminal repeat is removed, the
adenovirus chromosome is...
Ribonucleotide reductase is a remarkable enzyme that catalyzes the rate-limiting
step in the synthesis of the 2'-deoxynucleoside triphosphates. The intent of this project
was to characterize the ribonucleotide reductase encoded by the orthopoxvirus,
vaccinia. The first objective was to study the structural and functional features of the
viral small subunit...