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Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures

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dc.creator Larkin, Andrew
dc.creator Siddens, Lisbeth K.
dc.creator Krueger, Sharon K.
dc.creator Williams, David E.
dc.creator Baird, William M.
dc.date.accessioned 2011-06-29T18:49:15Z
dc.date.available 2011-06-29T18:49:15Z
dc.date.issued 2010
dc.identifier.uri http://hdl.handle.net/1957/21884
dc.description.abstract Epidermal RNA samples from FVB/N mice treated with PAH standards and environmental PAH mixtures were harvested and purified using Trizol extraction and RNeasy mini prep kit purification. Epidermal RNA integrity was low (RIN mean 3.5), indicating significant RNA degradation and the need to modify handling, collection, and processing of skin to limit degradation. Potential sources of RNA degrdation include RNase activity, both endogenous and exogenous, thermal degradation during heat-induced epidermal-dermal seperation, and contamination of stock solutions. Testing of the modified method produced dermal RNA samples with acceptable RIN integrity levels (RIN mean 7.6). Variations of the modified method were performed and compared to one another for method optimization. en_US
dc.language.iso en_US en_US
dc.subject Carcinogenesis en_US
dc.subject Carcinoma en_US
dc.subject Dermis en_US
dc.subject Gene expression en_US
dc.subject Polyclic aromatic hydrocarbons skin en_US
dc.title Method development for extraction and purification of dermal RNA from FVB/N mice treated with environmental PAH mixtures en_US
dc.type Research Paper en_US
dc.description.peerreview no en_US


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