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Acetylene Inhibition of Azotobacter vinelandii Hydrogenase: Acetylene Binds Tightly to the Large Subunit

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dc.creator Sun, Jin-Hua
dc.creator Hyman, Michael R.
dc.creator Arp, Daniel J.
dc.date.accessioned 2011-08-08T16:34:34Z
dc.date.available 2011-08-08T16:34:34Z
dc.date.issued 1992
dc.identifier.citation Sun, J., Hyman, M. R., & Arp, D. J. (1992). Acetylene Inhibition of Azotobacter vinelandii Hydrogenase: Acetylene Binds Tightly to the Large Subunit. Biochemistry, 31(12), 3158-3165. doi:10.1021/bi00127a016 en_US
dc.identifier.uri http://hdl.handle.net/1957/22429
dc.description.abstract Acetylene is a slow-binding inhibitor of the Ni- and Fe-containing dimeric hydrogenase isolated from Azotobacter vinelandii. Acetylene was released from hydrogenase during the recovery from inhibition. This indicates that no transformation of acetylene to another compound occurred as a result of the interaction with hydrogenase. However, the release of C2H2 proceeds more rapidly than the recovery of activity, which indicates that release of C2H2 is not sufficient for recovery of activity. Acetylene binds tightly to native hydrogenase; hydrogenase and radioactivity cuelute from a gel permeation column following inhibition with 14C2H2A. cetylene, or a derivative, remains bound to the large 65 000 MW subunit (and not to the small 35 000 MW subunit) of hydrogenase following denaturation as evidenced by SDS-PAGE and fluorography of l4C2H,-inhibited hydrogenase. This result suggests that C2H2, and by analogy H,, binds to and is activated by the large subunit of this dimeric hydrogenase. Radioactivity is lost from 14C2H,-inhibited protein during recovery. The inhibition is remarkably specific for C2H2: propyne, butyne, and ethylene are not inhibitors. en_US
dc.language.iso en_US en_US
dc.publisher American Chemical Society en_US
dc.relation.ispartofseries Biochemistry en_us
dc.relation.ispartofseries Vol. 31 (1992)
dc.subject Acetylene inhibition en_US
dc.subject Azotobacter vinelandii en_US
dc.title Acetylene Inhibition of Azotobacter vinelandii Hydrogenase: Acetylene Binds Tightly to the Large Subunit en_US
dc.type Article en_US
dc.description.peerreview yes en_US


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