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Isolation, extraction, and fluorometric measurements of nucleic acids and DNA photorepair in marine macrophytes

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dc.contributor.advisor Carlson, David J.
dc.creator Baeuerle, Brigitte G.
dc.date.accessioned 2011-09-20T17:45:16Z
dc.date.available 2011-09-20T17:45:16Z
dc.date.copyright 1990-12-10
dc.date.issued 1990-12-10
dc.identifier.uri http://hdl.handle.net/1957/23421
dc.description Graduation date: 1991 en_US
dc.description.abstract Nucleic acid estimates in tissues of marine macrophytes with the fluorochromes Hoechst 33258 and ethidium homodimer were severely impaired by high concentrations of interfering compounds. The isolation and extraction techniques used pointed towards compounds of low molecular weight and of polar characteristics that were insensitive to organic solvent extraction and enzymatic degradation. Interference was therefore thought to primarily arise from high concentrations of phenols commonly found in many seaweeds, which might prevent successful fluorochrome binding by complex formation with nucleic acids. None of the investigated isolation methods was sufficiently effective to allow accurate estimates of total nucleic acid content or to reliably establish the metabolic status of seaweeds by RNA/DNA ratios. Despite the uncertainties involved in nucleic acid estimations, DNA isolated by size exclusion chromatography was successfully used to show preliminary evidence for photo-activated DNA repair in marine macrophytes. en_US
dc.language.iso en_US en_US
dc.subject.lcsh Marine algae -- Oregon en_US
dc.subject.lcsh Nucleic acids -- Separation en_US
dc.title Isolation, extraction, and fluorometric measurements of nucleic acids and DNA photorepair in marine macrophytes en_US
dc.type Thesis/Dissertation en_US
dc.degree.name Master of Science (M.S.) in Oceanography en_US
dc.degree.level Master's en_US
dc.degree.discipline Oceanic and Atmospheric Sciences en_US
dc.degree.grantor Oregon State University en_US
dc.contributor.committeemember Taghon, Gary
dc.contributor.committeemember Schaup, Henry
dc.contributor.committeemember Taylor, Edward
dc.description.digitization File scanned at 300 ppi (Monochrome) using ScandAll PRO 1.8.1 on a Fi-6770A in PDF format. CVista PdfCompressor 4.0 was used for pdf compression and textual OCR. en_US
dc.description.peerreview no en_us

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