Abstract:
Excised apical stem tips (2-5 mm) of grape cultivars grew well
on Murashige and Skoog's (1962) medium for tobacco callus. Additions
of glycine were not essential but gave a slight stimulus to growth.
Naphthalene acetic acid slightly reduced growth rates but reductions
were not statistically significant. Kinetin and coconut milk were both
significantly detrimental to the growth of the excised tips. Tips that
developed callus following treatment with IBA (indolebutyric acid)
made more growth (significant at .01 probability) than tips without a
callus mass at the cut end. Indolebutyric acid was necessary to root
production in the second sub-culture on agar medium. Elongated stem
tips with roots, transferred to a liquid medium on a filter paper
bridge, grew vigorously and produced abundant roots. These young
plants were later transferred to greenhouse soils and developed to
mature, healthy plants typical of the cultivar.
