Graduate Thesis Or Dissertation

 

Isozyme banding patterns and embryo development in interspecific crosses of Phaseolus Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/4t64gr30n

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  • Interspecific hybridization between Phaseolus vulgaris and P. coccineus results in the formation of either mature F₁ seeds or abnormal embryos, depending on the direction of the cross. However, normal as well as abnormal embryos occur in the reciprocal F₂ populations. The exact cause of abnormal embryo development is unknown. To determine if there is a correlation between the genomic composition and embryo development (normal vs. abnormal), isozyme banding patterns were examined in the reciprocal F₁ and F₂ progenies of P. vulgaris cv. Great Northern (GN) and P. coccineus cv. Scarlet Runner (SR). Three enzyme systems, acid phosphatase (AP), esterase (EST), and malate dehydrogenase (MDH) were analysed in extracts of immature embryos at the cotyledonary stage. Parental species and reciprocal F₁ and F₂ embryos exhibited clear differences in electrophoretic patterns. The embryos of reciprocal crosses displayed banding patterns characteristic for their developmental type (normal or abnormal). AP and MDH zymograms of normal embryos were closer to those of the GN parent, whereas abnormal embryos had isozyme bands similar to those of SR. Differences in EST banding patterns were apparent between normal and abnormal F₂ embryos, however, these patterns did not resemble either parent. The results indicate that embryo development can be correlated with particular isozyme patterns which may at least in part reflect the genetic combination. They also seem to substantiate the hypothesis that the skewed distributions of quantitative traits in interspecific progeny populations could be related to selective survival of seeds containing normal embryos.
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