Graduate Thesis Or Dissertation

 

The role of glutathione in the development and release of rest on 'Redhaven' peaches Public Deposited

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https://ir.library.oregonstate.edu/concern/graduate_thesis_or_dissertations/2801pk46b

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  • A rapid and sensitive HPLC method for quantifying both the reduced and oxidized forms of glutathione simultaneously in woody plant tissues was developed. This method was applicable to a variety of woody plant species and different tissues. Total glutathione content in the bark tissue of seven of the woody plant species tested were lower during active plant growth, and higher in the dormant stage. All tested tissues of peach plants contained glutathione. The highest and lowest content was found in the leaves and roots, respectively. 'Redhaven' peach trees acquired the onset of rest on 1 Oct, before any chilling had been experienced. Maximum rest was reached on 1 Nov after the plants were exposed to 320 CU, and 50% of the buds were broken at 860 CU on 1 Dec. Total budbreak and phytotoxicity induced by hydrogen cyanamide applied to 'Redhaven' peach trees was dependent on concentration and time of application. Phytotoxicity was evident at all application dates, but greatest at the highest concentrations. Plants were more resistant to hydrogen cyanamide at maximum rest. Hydrogen cyanamide induced budbreak was best during the post-rest period. Budbreak and phytotoxicity induced by hydrogen cyanamide applied during the quiescent stage were dependent on posttreatment temperature and concentration. All cyanamide treatments inhibited budbreak at all tested temperatures. The inhibition was greatest at the the lowest temperature and at the highest concentrations. Phytotoxicity was greater at 15C and 35C than at 25C, and increased with increased concentrations. The content of GSH and GSSG in the buds changed with the development of rest. During the early phases of rest, when rest intensity increased, the content of GSH and GSSG decreased (15 Oct) and then increased at maximum rest (1 Nov). Content of GSH continued to increase and peaked on 1 Dec at 860 CU. On 15 Dec when the buds were qxiiescent GSH content decreased. In contrast, contents of GSSG did not drop during cjuiescence and were highest on 15 Dec. GSH content induced by chilling was closely associated with the end of rest. Hydrogen cyanamide treatments caused a rapid decrease of GSH content within 12 hr followed by a large increase 24 hr after application. The changes of GSH induced were inversely proportional to cyanamide concentration. Non-treated plants showed at relatively constant GSH and GSSG content during this period of time. Hydrogen cyanamide treatments increased GSH contents at all application dates. The extent of GSH changes was dependent on the physiological status of the bud and cyanamide concentration. At maximum rest the plants were more resistant to cyanamide and this coincided with the highest induced GSH content as compared to all application dates.
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